Document Detail


Beneficial effects of beta-sitosterol on glucose and lipid metabolism in L6 myotube cells are mediated by AMP-activated protein kinase.
MedLine Citation:
PMID:  18992226     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AMP-activated protein kinase (AMPK) is an energy-sensing enzyme that has been implicated as a key factor for controlling intracellular lipids and glucose metabolism. Beta-sitosterol, a plant sterol known to prevent cardiovascular disease was identified from Schizonepeta tenuifolia to an AMPK activator. In L6 myotube cells, beta-sitosterol significantly increased phosphorylation of the AMPKalpha subunit and acetyl-CoA carboxylase (ACC) with stimulating glucose uptake. In contrast, beta-sitosterol treatment reduced intracellular levels of triglycerides and cholesterol in L6 cells. These effects were all reversed by pretreatment with AMPK inhibitor Compound C or LKB1 destabilizer radicicol. Similarly, beta-sitosterol-induced phosphorylation of AMPK and ACC was not increased in HeLa cells lacking LKB1. These results together suggest that beta-sitosterol-mediated enhancement of glucose uptake and reduction of triglycerides and cholesterol in L6 cells is predominantly accomplished by LKB1-mediated AMPK activation. Our findings further reveal a molecular mechanism underlying the beneficial effects of beta-sitosterol on glucose and lipid metabolism.
Authors:
Seung-Lark Hwang; Ha-Na Kim; Hyun-Hee Jung; Ji-Eun Kim; Dong-Kyu Choi; Jong-Moon Hur; Jai-Youl Lee; Hebok Song; Kyung-Sik Song; Tae-Lin Huh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-04
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  377     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-28     Completed Date:  2008-12-16     Revised Date:  2012-06-22    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1253-8     Citation Subset:  IM    
Affiliation:
TG Biotech Research Institute, Technobuilding, Kyungpook National University, Daegu 702-831, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
AMP-Activated Protein Kinases / metabolism*
Animals
Cell Line
Cholesterol / metabolism
Enzyme Activation
Glucose / metabolism*
Glucose Transporter Type 4 / metabolism
Humans
Hypolipidemic Agents / pharmacology*
Lipid Metabolism / drug effects*
Muscle Fibers, Skeletal / drug effects*,  metabolism
Protein Transport / drug effects
Protein-Serine-Threonine Kinases / metabolism
Rats
Signal Transduction / drug effects
Sitosterols / pharmacology*
Triglycerides / metabolism
Chemical
Reg. No./Substance:
0/Glucose Transporter Type 4; 0/Hypolipidemic Agents; 0/Sitosterols; 0/Triglycerides; 50-99-7/Glucose; 57-88-5/Cholesterol; 5779-62-4/sitosterol; EC 2.7.11.1/AMP-Activated Protein Kinases; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/Stk11 protein, rat

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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