Document Detail


Beneficial effects of BAY u3405, a novel thromboxane A2 receptor antagonist, in splanchnic artery occlusion shock.
MedLine Citation:
PMID:  7878075     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Splanchnic artery occlusion shock was induced in male anaesthetized rats by clamping the splanchnic artery for 45 min. The arteries were then released and survival rate, mean survival time, mean arterial blood pressure, plasma levels of thromboxane B2 and 6-keto-PGF1 alpha, macrophage phagocytosis activity and plasma levels of myocardial depressant factor were evaluated. In addition, the neutrophilic infiltrate was quantified in the ileum and lung using a myeloperoxidase (MPO) assay. Sham splanchnic-artery-occlusion-shocked rats were used as controls. Splanchnic-artery-occlusion-shocked rats died within 93 +/- 7 min, while all sham-shocked animals survived more than 3 h. Splanchnic artery occlusion shock caused changes in mean arterial blood pressure, significantly increased the plasma levels of thromboxane B2 (7.5 +/- 1.3 ng/ml; p < 0.001 vs. sham), 6-keto-PGF1 alpha (8.9 +/- 1.7 ng/ml; p < 0.001 vs. sham) and myocardial depressant factor (114 +/- 11 U/ml), and reduced macrophage phagocytosis. Furthermore, MPO activity was significantly elevated (0.12 +/- 0.03 x 10(-3) and 1.8 +/- 0.5 x 10(-3) U/g protein in the ileum and lung, respectively) 70 min after starting reperfusion. Administration of BAY u3405, a novel thromboxane A2 receptor antagonist (30 mg/kg i.v., 30 min before occlusion), significantly increased survival time (187 +/- 3.7 min) and survival rate, improved mean arterial blood pressure, reduced the plasma levels of myocardial depressant factor (54 +/- 3 U/ml), partially restored macrophage phagocytosis and lowered MPO activity in both the ileum and the lung. Our data are consistent with an involvement of thromboxane A2 in splanchnic artery occlusion shock and suggest that BAY u3405 might be of benefit in low-flow states such as circulatory shock.
Authors:
P Canale; F Squadrito; D Altavilla; M Ioculano; G M Campo; G Squadrito; G Urna; A Sardella; A P Caputi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pharmacology     Volume:  49     ISSN:  0031-7012     ISO Abbreviation:  Pharmacology     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-04-03     Completed Date:  1995-04-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0152016     Medline TA:  Pharmacology     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  376-85     Citation Subset:  IM    
Affiliation:
Institute of Pharmacology, School of Medicine, University of Messina, Italy.
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MeSH Terms
Descriptor/Qualifier:
6-Ketoprostaglandin F1 alpha / blood
Animals
Arterial Occlusive Diseases / drug therapy*,  mortality,  physiopathology
Blood Pressure / physiology
Carbazoles / pharmacology*,  therapeutic use
Macrophages / physiology
Male
Myocardial Depressant Factor / blood
Peroxidase / metabolism
Phagocytosis / physiology
Rats
Rats, Sprague-Dawley
Receptors, Thromboxane / antagonists & inhibitors*
Shock / drug therapy*,  physiopathology
Splanchnic Circulation / drug effects*
Sulfonamides / pharmacology*,  therapeutic use
Survival Rate
Thromboxane A2 / antagonists & inhibitors*
Thromboxane B2 / blood
Chemical
Reg. No./Substance:
0/Carbazoles; 0/Myocardial Depressant Factor; 0/Receptors, Thromboxane; 0/Sulfonamides; 116649-85-5/ramatroban; 54397-85-2/Thromboxane B2; 57576-52-0/Thromboxane A2; 58962-34-8/6-Ketoprostaglandin F1 alpha; EC 1.11.1.7/Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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