Document Detail


Beneficial effect of steroid on myocardial preservation in isolated rat hearts.
MedLine Citation:
PMID:  8788373     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Myocardial injury after heart storage has been known to contribute to an increase in myocardial calcium content or oxygen free radical production. The present experiment was designed to determine the inhibitory effect of steroid in the preservation solution on myocardial injury during simple immersion of isolated rat hearts. The experimental group was divided into 5 sub-groups according to the concentration of steroid added to the solution. Group I was stored in preservation solution containing no steroid. Groups II to V were stored in preservation solutions containing methylprednisolone in concentrations of 50 mg/L, 100 mg/L, 150 mg/L, and 200 mg/L, respectively. After the rat hearts were isolated, crystalloid cardioplegic solution at 4 degrees C was used for cardiac arrest, and the hearts were immersed in 4 degrees C Euro-Collins solution for 8 h. Hearts were then maintained under ischemic conditions at 25 degrees C for 15 min. After 60 min of reperfusion at 37 degrees C, cardiac function was measured and biochemical analyses of coronary effluent and myocardium were performed. The cardiac outputs in groups IV and V were significantly higher than those in groups I and II (p < 0.01). Creatine kinase-MB in coronary effluent in groups IV and V were significantly lower than those in groups I and II at 15, 30 and 60 min after reperfusion (p < 0.05). The level of arachidonic acid at 1 and 5 min, and the level of malondialdehyde at 5 min, after reperfusion in groups III, IV and V were significantly lower than those in groups I and II. Myocardial adenosine triphosphate was most preserved in group IV, and myocardial arachidonic acid, malondialdehyde, and calcium levels and water content in groups IV and V were lower than those in groups I and II. Ultrastructural observations also showed that myofilaments and mitochondria were well preserved in groups III, IV and V at 15 min after reperfusion compared with those in groups I and II. These results indicate that the addition of steroid to preservation solution might be effective in preventing myocardial injury during preservation of rat hearts, and the optimal concentration appears to range from 150 mg/L to 200 mg/L.
Authors:
K Hisatomi; T Isomura; T Sato; N Hayashida; K Kosuga; K Ohishi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Japanese circulation journal     Volume:  59     ISSN:  0047-1828     ISO Abbreviation:  Jpn. Circ. J.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-11-21     Completed Date:  1996-11-21     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7806868     Medline TA:  Jpn Circ J     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  815-23     Citation Subset:  IM    
Affiliation:
Second Department of Surgery, Kurume University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Animals
Anti-Inflammatory Agents / pharmacology*
Arachidonic Acids / metabolism
Cardiac Output / drug effects
Creatine Kinase / metabolism
Heart / drug effects*,  physiology
Isoenzymes
Male
Malondialdehyde / metabolism
Methylprednisolone / pharmacology*
Microfilaments / ultrastructure
Mitochondria, Heart / ultrastructure
Myocardium / metabolism*,  ultrastructure
Organ Preservation*
Rats
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Arachidonic Acids; 0/Isoenzymes; 542-78-9/Malondialdehyde; 56-65-5/Adenosine Triphosphate; 83-43-2/Methylprednisolone; EC 2.7.3.2/Creatine Kinase

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