Document Detail


Beneficial effects of reversine on in vitro development of miniature pig somatic cell nuclear transfer embryos.
MedLine Citation:
PMID:  20103988     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reversine, a 2-(4-morpholinoanilino)-6-cyclohexylaminopurine analog, can induce dedifferentiation of myogenic lineage-committed cells into multipotent mesenchymal progenitor cells, from which osteoblasts and adipocytes redifferentiate under lineage-specific inducing conditions. Although the molecular mechanism of how reversine causes dedifferentiation of a differentiated cell has not been fully elucidated, we speculated that it would be involved in reprogramming. In the present study, we examined whether reversine can enhance the development of somatic cell nuclear transfer (SCNT) embryos by improving the reprogramming state of the somatic cell nuclei. As donor cells, we used miniature pig fetal fibroblasts transfected with a plasmid construct containing a mouse Oct-3/4 promoter and enhanced green fluorescent protein (EGFP) cDNA. When the nuclei of these transfected cells are reprogrammed to an undifferentiated state in the SCNT embryos, EGFP expression is expected to commence under the control of the Oct-3/4 promoter. After SCNT, the resulting embryos were treated with 5 muM reversine for different durations (0, 6, 12, 18 and 24 h) or at different concentrations (0, 1, 5 and 10 muM) of reversine for 12 h and then cultured in vitro. When embryos were treated with 5 muM reversine for 12 h, the blastocyst formation rate was significantly (P<0.01) higher than that of embryos without reversine treatment. However, the strength and pattern of EGFP expression in the embryos were not affected by the same treatment. A normal-looking fetus was obtained 21 days after transfer of embryos treated with 5 muM reversine for 12 h into recipients. The present findings indicate that treatment with reversine is beneficial for enhancement of the in vitro development of miniature pig SCNT embryos, although the underlying mechanism is still unclear.
Authors:
Kazuchika Miyoshi; Hironori Mori; Yamato Mizobe; Takehiro Himaki; Mitsutoshi Yoshida; Masahiro Sato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-27
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  56     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-05-14     Completed Date:  2010-06-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  291-6     Citation Subset:  IM    
Affiliation:
Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan. kmiyoshi@agri.kagoshima-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Embryo Culture Techniques / methods*,  veterinary*
Embryonic Development / drug effects,  physiology
Female
Fertilization in Vitro / methods,  veterinary
Morpholines / pharmacology*
Nuclear Transfer Techniques / veterinary*
Oocytes / physiology
Pregnancy
Purines / pharmacology*
Swine
Swine, Miniature / embryology*
Chemical
Reg. No./Substance:
0/2-(4-morpholinoanilino)-6-cyclohexylaminopurine; 0/Morpholines; 0/Purines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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