Document Detail

Beige granules as a cell marker for clonal analysis in kidney and liver of mouse aggregation chimaeras, and three-dimensional reconstruction from serial paraffin sections.
MedLine Citation:
PMID:  3256469     Owner:  NLM     Status:  MEDLINE    
Anomalous giant granules of beige (bg) mice have been used as a cell marker in the study of cell lineage of mast cells. Similar granules are known to exist in other tissues including kidney proximal tubules and liver parenchymal cells. In the present study, these granules were found to give yellow or orange autofluorescence when the tissue had been fixed with formaldehyde and embedded in paraffin. Thus, the granules can be used as a cell marker that can be visualized in serial paraffin sections without any specific histochemical staining. Chimaeric mice were produced by aggregation of 8-cell-stage embryos of beige (C57BL/6J-bgJ/bgJ) and A/J strains. The chimaeric liver showed beige cell patches with complicated shapes, although the patches frequently conformed to the shape of parenchymal cell cord or plate structures. In chimaeric kidney, beige cells formed coherent patches in the proximal tubules. The tubules were found to contain more than one clone. The patches frequently had long extended shapes suggesting growth of the clone along the tubule axis. Three-dimensional image reconstruction from the serial paraffin sections was carried out with the aid of a computer-assisted image analysis system, resulting in a clearer image of the patch shape.
N Nakatsuji
Related Documents :
3391369 - Secretin empties bile duct cell cytoplasm of vesicles when it initiates ductular hco3- ...
3046959 - Localization of brain type creatine kinase in kidney epithelial cell subpopulations in ...
6532039 - Incidence and significance of ultrastructural changes in the common bile duct in lithia...
15102609 - Uptake and trafficking of fluorescent conjugates of folic acid in intact kidney determi...
53169 - Studies on vertebrate kidney. v. pas-positive lipids in kidneys of fishes from differen...
23645379 - On pseudo-onion bulb intraneural proliferations of the non-major nerves of the oral muc...
14705939 - Using nanoparticle optics assay for direct observation of the function of antimicrobial...
2458799 - Expression of neurofilament proteins by purkinje cells: ultrastructural immunolocalizat...
539589 - A new treatment strategy for hemophilia b: incorporation of factor ix into red cell gho...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  104     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1989-10-26     Completed Date:  1989-10-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  383-90     Citation Subset:  IM    
Division of Developmental Biology, Meiji Institute of Health Science, Odawara, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Clone Cells
Image Processing, Computer-Assisted
Kidney Tubules / cytology*,  ultrastructure
Liver / cytology*,  ultrastructure
Mast Cells / physiology*
Mice, Mutant Strains
Microscopy, Fluorescence

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Patterns of fibronectin gene expression and splicing during cell migration in chicken embryos.
Next Document:  The localization of laminin mRNA and protein in the postimplantation embryo and placenta of the mous...