Document Detail


Behaviour of a peptide sequence from the GB virus C/hepatitis G virus E2 protein in Langmuir monolayers: its interaction with phospholipid membrane models.
MedLine Citation:
PMID:  19232456     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The GB virus C/hepatitis G virus (GBV C/HGV) is a Flaviviridae member that despite its non pathogenicity, has become of great interest given that it could inhibit the replication of the human immunodeficiency virus (HIV). Therefore, a better knowledge of the virus peptides involved in the cellular membrane fusion mechanism has become our aim. The selected peptide, named E2(347-363), corresponds to the GBV-C/HGV E2 protein and has been synthesized in order to study its interaction with in vitro membrane models. Two phospholipids, varying the charge and the unsaturations of the hydrocarbon chain have been chosen: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (DOPG). For our porpoise, we have used the Langmuir monolayer technique and Brewster angle microscopy (BAM) to gain deeper insight into the peptide/lipid interactions. The results obtained allow us to argue in favour of considering E2(347-363) a success candidate for developing further experiments in order to determine its potential role in the GBV C/HGV virus/cell membrane fusion process.
Authors:
Silvia Pérez-López; Marina Nieto-Suárez; Concepció Mestres; M Asunción Alsina; Isabel Haro; Nuria Vila-Romeu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-30
Journal Detail:
Title:  Biophysical chemistry     Volume:  141     ISSN:  1873-4200     ISO Abbreviation:  Biophys. Chem.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-03-24     Completed Date:  2009-06-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  153-61     Citation Subset:  IM    
Affiliation:
Department of Physical Chemistry, Faculty of Sciences, University of Vigo, Campus Universitario As Lagoas s/n 32004 Ourense, Spain.
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MeSH Terms
Descriptor/Qualifier:
1,2-Dipalmitoylphosphatidylcholine / metabolism*
GB virus C
Membranes, Artificial*
Models, Molecular
Peptide Fragments / metabolism*
Phosphatidylglycerols / metabolism*
Pressure
Surface Properties
Viral Envelope Proteins / chemical synthesis,  metabolism*
Chemical
Reg. No./Substance:
0/Membranes, Artificial; 0/Peptide Fragments; 0/Phosphatidylglycerols; 0/Viral Envelope Proteins; 0/glycoprotein E2, GB virus C; 2644-64-6/1,2-Dipalmitoylphosphatidylcholine; 66322-31-4/1,2-dioleoyl-sn-glycero-3-phosphoglycerol

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