| Behavior of synthetic polymers immobilized on a cell membrane. | |
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MedLine Citation:
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PMID: 18191192 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We used three kinds of polymers that interact with living cells in different modes: poly(ethylene glycol)-conjugated phospholipid (PEG-lipid) and poly(vinyl alcohol) carrying alkyl side chains (PVA-alkyl), expected to anchor to the membrane lipid bilayer through hydrophobic interactions; N-hydroxysuccinimidyl-PEG (PEG-NHS), which covalently bonds with all kinds of membrane proteins having amino groups on cell surfaces; and polyelectrolytes, poly(ethylene imine) (PEI) and carboxylated PVA (PVA-COOH), which interact with cells electrostatically. CCRF-CEM (T-cell like) and HEK293 (adherent cell) cell lines were used. We followed the surface dynamics of fluorescently labeled polymers on living cells over time using confocal laser scanning microscopy and flow cytometry. PEI destroyed cells, while PVA-COOH did not interact with cells. PEG-lipid, PVA-alkyl, and PEG-NHS interacted with cells without cytotoxicity and existed on the cell surface even after cells were washed. PEG-lipid and PEG-NHS were rapidly excluded from the cell surface without cytoplasmic uptake, while PVA-alkyl assembled on the living cell surface was taken into the cytoplasm and then excluded. Most polymers were excluded within 24h although exclusion routes seemed to be different between polymers, suggesting that cell transplant surface modifications are shorter than has been assumed. The short life of modified polymers on the cell surface should be a consideration for cell transplant surface modifications. |
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Authors:
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Yuji Teramura; Yoshihiro Kaneda; Takahiko Totani; Hiroo Iwata |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-11 |
Journal Detail:
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Title: Biomaterials Volume: 29 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-01-29 Completed Date: 2008-07-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 1345-55 Citation Subset: IM |
Affiliation:
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Department of Nano-Medicine Merger Education Unit, Kyoto University, 53 Kawara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Cell Membrane / chemistry* Flow Cytometry Humans Hydrophobicity Lipid Bilayers / chemistry Microscopy, Confocal Models, Biological Molecular Structure Polyethylene Glycols / chemical synthesis, chemistry Polyethyleneimine / chemical synthesis, chemistry Polymers / chemical synthesis, chemistry* Polyvinyl Alcohol / chemical synthesis, chemistry |
| Chemical | |
Reg. No./Substance:
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0/Lipid Bilayers; 0/Polyethylene Glycols; 0/Polymers; 9002-89-5/Polyvinyl Alcohol; 9002-98-6/Polyethyleneimine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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