Document Detail


Behavior of mouse spermatogonial stem-like cells on an electrospun nanofibrillar matrix.
MedLine Citation:
PMID:  23274510     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Spermatogonial stem cells are affected by the interactions of extrinsic signals produced by components of the microenvironment niche, in addition to the chemical and physical properties of the extracellular matrix. Therefore, this study was initiated to assess the interaction of these cells on a synthetic nanofibrillar extracellular matrix that mimicked the geometry and nanotopography of the basement membrane for cellular growth.
METHODS: This study has used a variety of experimental approaches to investigate the interaction of mouse neonatal-derived spermatogonial stem-like cells on a synthetic random oriented three-dimensional nanofibrillar matrix composed of electrospun polyamide nanofibers (Ultra-Web™).
RESULTS: Spermatogonial stem-like cell colonies were characterized by their ability to express α6-integrin, Thy-1, PLZF, and β1-integrin. After culture of cells on the nanofibrillar surfaces for 7 days, the number of colonies, the number of cells in each colony, and the average area of colonies were increased (P < 0.05). However, the expression difference of related markers in both groups was not significant. A significantly higher proliferation and survival was observed in the nanofibrillar group (P < 0.05). After transplantation into the testes of busulfan-treated adult mice, spermatogonial stem-like cell colonies that were cultured on the nanofibrillar surface demonstrated functionality, as verified by their ability to migrate to the seminiferous basal membrane, where they produced additional colonies.
CONCLUSIONS: These results have suggested that electrospun nanofibrillar surfaces could provide a more favorable microenvironment for in vitro short term culture of spermatogonial stem-like cell colonies.
Authors:
Malak Shakeri; Hamid Kohram; Abdolhossein Shahverdi; Ahmad Zare Shahneh; Faranak Tavakolifar; Mehdi Pirouz; Hossein Moradi Shahrebabak; Morteza Koruji; Hossein Baharvand
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-12-30
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  30     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-26     Completed Date:  2013-09-23     Revised Date:  2014-03-07    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  325-32     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / methods*
Cell Differentiation
Cell Proliferation
Extracellular Matrix / chemistry,  metabolism
Female
Male
Mice
Nanofibers / chemistry*
Pregnancy
Spermatogonia / cytology*
Stem Cells / cytology*,  metabolism
Surface Properties
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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