Document Detail

Behavior and characteristics of biogenic amines, ornithine and lysine derivatized with the o-phthalaldehyde-ethanethiol-fluorenylmethyl chloroformate reagent.
MedLine Citation:
PMID:  16130716     Owner:  NLM     Status:  MEDLINE    
The stability and characteristics of the ornithine (Orn), lysine (Lys), putrescine (Put), cadaverine (Cad), 1,7-diaminoheptane (Diah), spermidine (Spd) and spermine (Spm) derivatives obtained with the o-phthalaldehyde (OPA)-ethanethiol (ET)-fluorenylmethyl chloroformate (FMOC) reagent has been investigated. The stoichiometry of the introduced, two-step derivatization process has been followed by photodiode array (DAD) and fluorescence (FL) detections, simultaneously, while the composition of derivatives was confirmed by on-line HPLC-electrospray ionization (ESI) MS measurements. Depending on the composition of the OPA reagents, in addition to the secondary amino group-containing Spd and Spm, under common aqueous conditions also Orn and Lys do react with FMOC resulting in derivatives of various compositions. Applying the OPA-ET reagent of increasing methanol (Met) content (38-80%, v/v) the formation of the FMOC group containing Orn and Lys derivatives could be considerably decreased. Optimum elution condition (18 min, including equilibration) was developed for the simultaneous quantitation of Orn, Lys, Put, Cad, Diah, Spd and Spm, in the presence of the rest of protein amino acids. The practical utility of the method was demonstrated by the analysis of mouse tissues. Average reproducibility of quantitations, characterized with the relative standard deviation percentages of fluorescence intensities and UV responses, in order of listing, proved to be 2.1% and 2.1%, respectively.
R Hanczko; A Korös; F Tóth; I Molnár-Perl
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1087     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-31     Completed Date:  2005-10-28     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  210-22     Citation Subset:  IM    
Institute of Inorganic and Analytical Chemistry, L. Eötvös University, H-1518, Budapest 112, P.O. Box 32, Hungary.
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MeSH Terms
Biogenic Amines / chemistry*
Chromatography, High Pressure Liquid
Fluorenes / chemistry*
Formic Acids / chemistry*
Lysine / chemistry*
Ornithine / chemistry*
Reference Standards
Spectrometry, Mass, Electrospray Ionization
Sulfhydryl Compounds / chemistry*
o-Phthalaldehyde / chemistry*
Reg. No./Substance:
0/Biogenic Amines; 0/Fluorenes; 0/Formic Acids; 0/Sulfhydryl Compounds; 56-87-1/Lysine; 643-79-8/o-Phthalaldehyde; 7006-33-9/Ornithine; 75-08-1/ethanethiol
Erratum In:
J Chromatogr A. 2005 Nov 11;1094(1-2):201

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