Document Detail


Begomovirus coat protein interacts with a small heat-shock protein of its transmission vector (Bemisia tabaci).
MedLine Citation:
PMID:  19817909     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Tomato yellow leaf curl Sardinia virus (TYLCSV) is transmitted from plant to plant by the whitefly Bemisia tabaci in a persistent-circulative manner. The coat protein (CP) plays an important role in this transmission cycle. In this study, the CP was used to screen a Bemisia tabaci cDNA library using the yeast two-hybrid system, in a search for interacting partners. A member of the small heat-shock protein family (termed BtHSP16) was identified and its interaction with the CP was verified by an in vitro pull-down assay. The binding domain was located at the variable N-terminal part of the CP, while full-length BtHSP16 is required for the interaction. The putative role for this interaction in the transmission cycle by the whitefly is discussed.
Authors:
S Ohnesorge; E R Bejarano
Related Documents :
17066379 - A toxr-based dominant-negative system to investigate heterotypic transmembrane domain i...
18831549 - A global metabolite profiling approach to identify protein-metabolite interactions.
20580959 - Nanovesicle trapping for studying weak protein interactions by single-molecule fret.
10669729 - Complex protein interactions within the human polyadenylation machinery identify a nove...
18428659 - Split-ubiquitin system for identifying protein-protein interactions in membrane and ful...
15003599 - A two-step two-hybrid system to identify functionally significant protein-protein inter...
7765469 - Protein chaperones and protein folding.
1457049 - Preparative in vitro synthesis of bioactive human interleukin-2 in a continuous flow tr...
3769859 - In vitro kinetics of synthesis and release of mouse placental lactogen.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-08
Journal Detail:
Title:  Insect molecular biology     Volume:  18     ISSN:  1365-2583     ISO Abbreviation:  Insect Mol. Biol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9303579     Medline TA:  Insect Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  693-703     Citation Subset:  IM    
Affiliation:
Unidad de Genética, Departamento de Biología Celular, Genética y Fisiología, Universidad de Málaga, Málaga, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Juvenile polymyositis or paediatric muscular dystrophy: a detailed re-analysis of 13 cases.
Next Document:  Age- and division-of-labour-dependent differential expression of a novel non-coding RNA, Nb-1, in th...