Document Detail

Beetroot juice reduces infarct size and improves cardiac function following ischemia-reperfusion injury: Possible involvement of endogenous H2S.
MedLine Citation:
PMID:  25361774     Owner:  NLM     Status:  Publisher    
Ingestion of high dietary nitrate in the form of beetroot juice (BRJ) has been shown to exert antihypertensive effects in humans through increasing cyclic guanosine monophosphate (cGMP) levels. Since enhanced cGMP protects against myocardial ischemia-reperfusion (I/R) injury through upregulation of hydrogen sulfide (H2S), we tested the hypothesis that BRJ protects against I/R injury via H2S. Adult male CD-1 mice received either regular drinking water or those dissolved with BRJ powder (10 g/L, containing ∼0.7 mM nitrate). Seven days later, the hearts were explanted for molecular analyses. Subsets of mice were subjected to I/R injury by occlusion of the left coronary artery for 30 min and reperfusion for 24 h. A specific inhibitor of H2S producing enzyme - cystathionine-γ-lyase (CSE), dl-propargylglycine (PAG, 50 mg/kg) was given i.p. 30 min before ischemia. Myocardial infarct size was significantly reduced in BRJ-fed mice (15.8 ± 3.2%) versus controls (46.5 ± 3.5%, mean ± standard error [SE], n = 6/group, P < .05). PAG completely blocked the infarct-limiting effect of BRJ. Moreover, BRJ significantly preserved ventricular function following I/R. Myocardial levels of H2S and its putative protein target - vascular endothelial growth factor receptor 2 (VEGFR2) were significantly increased by BRJ intake, whereas CSE mRNA and protein content did not change. Interestingly, the BRJ-induced cardioprotection was not associated with elevated blood nitrate-nitrite levels following I/R nor induction of cardiac peroxiredoxin 5, a mitochondrial antioxidant enzyme previously linked to nitrate-induced cardioprotection. We conclude that BRJ ingestion protects against post-I/R myocardial infarction and ventricular dysfunction possibly through CSE-mediated endogenous H2S generation. BRJ could be a promising natural and inexpensive nutraceutical supplement to reduce cardiac I/R injury in patients.
Fadi N Salloum; Gregory R Sturz; Chang Yin; Shabina Rehman; Nicholas N Hoke; Rakesh C Kukreja; Lei Xi
Related Documents :
2816414 - Dissociation of cimetidine effects on inotropic and chronotropic action in cardiac anap...
20729274 - Expression of the anthracycline-metabolizing enzyme carbonyl reductase 1 in hearts from...
6508964 - Left ventricular bands. a normal anatomical feature.
1804854 - Ventilatory control of heart rate during inhalation of 5% co2 and types of panic attacks.
24668024 - Carvedilol induces greater control of β2- than β 1-adrenoceptor-mediated inotropic and ...
18650984 - Effect of acute subendocardial ischemia on ventricular refractory periods.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-10-30
Journal Detail:
Title:  Experimental biology and medicine (Maywood, N.J.)     Volume:  -     ISSN:  1535-3699     ISO Abbreviation:  Exp. Biol. Med. (Maywood)     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-11-1     Completed Date:  -     Revised Date:  2014-11-2    
Medline Journal Info:
Nlm Unique ID:  100973463     Medline TA:  Exp Biol Med (Maywood)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2014 by the Society for Experimental Biology and Medicine.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Implication of Bcl-2-associated athanogene 3 in fibroblast growth factor-2-mediated epithelial-mesen...
Next Document:  A homozygous splice-site mutation in CARS2 is associated with progressive myoclonic epilepsy.