| Bee venom induces apoptosis through intracellular Ca(2+) -modulated intrinsic death pathway in human bladder cancer cells. | |
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MedLine Citation:
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PMID: 22151644 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Objectives: To focus on bee venom-induced apoptosis in human bladder cancer TSGH-8301 cells and to investigate its signaling pathway to ascertain whether intracellular calcium iron (Ca(2+) ) is involved in this effect. Methods: Bee venom-induced cytotoxic effects, productions of reactive oxygen species and Ca(2+) and the level of mitochondrial membrane potential (ΔΨm) were analyzed by flow cytometry. Apoptosis-associated proteins were examined by Western blot analysis and confocal laser microscopy. Results: Bee venom-induced cell morphological changes and decreased cell viability through the induction of apoptosis in TSGH-8301 cell were found. Bee venom promoted the protein levels of Bax, caspase-9, caspase-3 and endonuclease G. The enhancements of endoplasmic reticulum stress-related protein levels were shown in bee venom-provoked apoptosis of TSGH-8301 cells. Bee venom promoted the activities of caspase-3, caspase-8, and caspase-9, increased Ca(2+) release and decreased the level of ΔΨm. Co-localization of immunofluorescence analysis showed the releases of endonuclease G and apoptosis-inducing factor trafficking to nuclei for bee venom-mediated apoptosis. The images revealed evidence of nuclear condensation and formation of apoptotic bodies by 4',6-diamidino-2-phenylindole staining and DNA gel electrophoresis showed the DNA fragmentation in TSGH-8301 cells. Conclusions: Bee venom treatment induces both caspase-dependent and caspase-independent apoptotic death through intracellular Ca(2+) -modulated intrinsic death pathway in TSGH-8301 cells. |
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Authors:
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Siu-Wan Ip; Yung-Lin Chu; Chun-Shu Yu; Po-Yuan Chen; Heng-Chien Ho; Jai-Sing Yang; Hui-Ying Huang; Fu-Shin Chueh; Tung-Yuan Lai; Jing-Gung Chung |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-11 |
Journal Detail:
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Title: International journal of urology : official journal of the Japanese Urological Association Volume: - ISSN: 1442-2042 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9440237 Medline TA: Int J Urol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 The Japanese Urological Association. |
Affiliation:
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Departments of Nutrition Biological Science and Technology Biochemistry Pharmacology School of Pharmacy, China Medical UniversityDepartments of Health and Nutrition Biotechnology Biotechnology, Asia University, Taichung Department of Chinese Medicine, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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