Document Detail


Beauvericin and ochratoxin A genotoxicity evaluated using the alkaline comet assay: single and combined genotoxic action.
MedLine Citation:
PMID:  20352195     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was aimed at investigating the genotoxic potential of single beauvericin (BEA) and ochratoxin A (OTA) as well as their interaction in porcine kidney epithelial PK15 cells and human leukocytes using the alkaline comet assay. IC(50) of BEA (5.0 +/- 0.6) and OTA (15.8 +/- 1.5) estimated by MTT reduction assay shows that BEA is three times more toxic than OTA. BEA (0.1 and 0.5 microM) and OTA (1 and 5 microM) were applied alone or in combination of these concentrations for 1 and 24 h in PK15 cells and human leukocytes. Genotoxicity of these toxins to PK15 cells was time- and concentration dependent. After 1 h, significant increase in tail length, tail intensity, tail moment, and abnormal sized tails (AST) was noted upon exposure to 1 muM of OTA alone and BEA + OTA combinations. Single BEA (0.5 microM) and OTA (1 and 5 microM) and their combinations evoked significant DNA damage in PK15 cells, considering all comet tail parameters measured after 24 h of treatment. Human leukocytes were slightly concentration but not time dependent. After 1 h of exposure, there were no significant changes in the tail length. Tail intensity, tail moment, and/or incidence of AST were significantly higher in cells treated with single OTA or BEA and their combinations than in control cells. DNA damage in leukocytes was significantly higher after 24 h of exposure to single toxins and their combinations, considering all comet tail parameters, but these changes were less pronounced than in PK15 cells. Combined toxins showed additive and synergistic effects in PK15 cells, while only additive effects were observed in human leukocytes. Combined prolonged exposure to BEA and OTA in subcytotoxic concentrations through food consumption could induce DNA damage contributing to the carcinogenicity in animals and humans.
Authors:
Maja Segvić Klarić; Dina Darabos; Ruzica Rozgaj; Vilena Kasuba; Stjepan Pepeljnjak
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-30
Journal Detail:
Title:  Archives of toxicology     Volume:  84     ISSN:  1432-0738     ISO Abbreviation:  Arch. Toxicol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-26     Completed Date:  2010-10-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417615     Medline TA:  Arch Toxicol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  641-50     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Schrottova 39, 10000 Zagreb, Croatia. msegvic@pharma.hr
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Survival / drug effects
Comet Assay
Depsipeptides / toxicity*
Drug Interactions
Humans
Kidney / drug effects,  metabolism
Leukocytes / drug effects,  metabolism
Mutagens / toxicity*
Ochratoxins / toxicity*
Swine
Toxicity Tests
Chemical
Reg. No./Substance:
0/Depsipeptides; 0/Mutagens; 0/Ochratoxins; 26048-05-5/beauvericin; 303-47-9/ochratoxin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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