| BeadCons: detection of nucleic acid sequences by flow cytometry. | |
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MedLine Citation:
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PMID: 18770821 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Molecular beacons are single-stranded nucleic acid structures with a terminal fluorophore and a distal, terminal quencher. These molecules are typically used in real-time PCR assays, but have also been conjugated with solid matrices. This unit describes protocols related to molecular beacon-conjugated beads (BeadCons), whose specific hybridization with complementary target sequences can be resolved by cytometry. Assay sensitivity is achieved through the concentration of fluorescence signal on discrete particles. By using molecular beacons with different fluorophores and microspheres of different sizes, it is possible to construct a fluid array system with each bead corresponding to a specific target nucleic acid. Methods are presented for the design, construction, and use of BeadCons for the specific, multiplexed detection of unlabeled nucleic acids in solution. The use of bead-based detection methods will likely lead to the design of new multiplex molecular diagnostic tools. |
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Authors:
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Douglas Horejsh; Federico Martini; Maria Rosaria Capobianchi |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.] Volume: Chapter 13 ISSN: 1934-9300 ISO Abbreviation: Curr Protoc Cytom Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2008-09-04 Completed Date: 2008-10-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100899351 Medline TA: Curr Protoc Cytom Country: United States |
Other Details:
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Languages: eng Pagination: Unit13.5 Citation Subset: IM |
Affiliation:
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National Institute for Infectious Diseases "L. Spallazani"-IRCSS, Rome, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence* DNA / chemistry*, genetics Flow Cytometry / methods* Fluorescent Dyes Nucleic Acid Conformation Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis / methods Oligonucleotide Probes Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Oligonucleotide Probes; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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