Document Detail


Bcr-Abl activates the AKT/Fox O3 signalling pathway to restrict transforming growth factor-beta-mediated cytostatic signals.
MedLine Citation:
PMID:  16113647     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fusion of Abl with either Bcr or Tel in human leukaemia leads to the constitutive activation of Abl tyrosine kinase, which in turn induces growth-factor-independent proliferation and cell survival. However, the mechanism by which Bcr-Abl induces cellular transformation has not yet been well characterized. Here, we show that Bcr-Abl-expressing cells are resistant to growth inhibition and apoptosis mediated by transforming growth factor-beta (TGF-beta). Interestingly, we observed that the suppressive effects of Bcr-Abl on TGF-beta responses were not mediated by an impairment of Smad signalling, which is believed to act as the principal mediator of TGF-beta responses. In contrast, we found that Bcr-Abl can target the protein kinase AKT and the transcription factor Fox O3 to interfere with growth inhibition and apoptosis in response to TGF-beta. Our results show a novel mechanism of cellular transformation by the oncogenic fusion protein Bcr-Abl through suppression of the cytostatic actions of TGF-beta.
Authors:
Azeddine Atfi; Lucile Abécassis; Marie-Francoise Bourgeade
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  EMBO reports     Volume:  6     ISSN:  1469-221X     ISO Abbreviation:  EMBO Rep.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-13     Completed Date:  2006-01-31     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100963049     Medline TA:  EMBO Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  985-91     Citation Subset:  IM    
Affiliation:
INSERM U482, 184 Rue du Faubourg St-Antoine, 75571 Paris, France. atfi@st-antoine.inserm.fr
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MeSH Terms
Descriptor/Qualifier:
1-Phosphatidylinositol 3-Kinase / physiology
Animals
Apoptosis
Blotting, Western
Cell Line
Cell Proliferation
Fluorescent Antibody Technique
Forkhead Transcription Factors / physiology*
Fusion Proteins, bcr-abl / physiology*
Humans
Mice
Phosphorylation
Proto-Oncogene Proteins c-akt / physiology*
Signal Transduction / physiology*
Transforming Growth Factor beta / biosynthesis,  physiology*
Chemical
Reg. No./Substance:
0/Forkhead Transcription Factors; 0/Fusion Proteins, bcr-abl; 0/Transforming Growth Factor beta; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.11.1/Proto-Oncogene Proteins c-akt
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