Document Detail


Bclx regulates the survival of double-positive thymocytes.
MedLine Citation:
PMID:  7761398     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The bclx gene has been shown to regulate programmed cell death in vitro. We now show that Bclx expression increases dramatically when T cells differentiate from CD4- CD8- (double negative) thymocytes to CD4+ CD8+ [double positive (DP)] thymocytes. In contrast single-positive (SP) thymocytes express negligible amounts of Bclx protein. This expression pattern contrasts with that of Bcl2, which is present in double-negative thymocytes, down-regulated in DP thymocytes, and reinduced upon maturation to SP thymocytes. Elimination of Bclx by gene targeting dramatically shortens the survival of DP thymocytes but not the survival of SP thymocytes or peripheral SP T cells. These data suggest that the induction of Bclx during thymic maturation plays a critical role in regulating the length of time DP thymocytes survive in the absence of selection.
Authors:
A Ma; J C Pena; B Chang; E Margosian; L Davidson; F W Alt; C B Thompson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  92     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-06-29     Completed Date:  1995-06-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4763-7     Citation Subset:  IM    
Affiliation:
Howard Hughes Medical Institute, Children's Hospital, Boston, MA, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Antigens, CD4 / analysis
Antigens, CD8 / analysis
Cell Death
Cell Survival / immunology
Cells, Cultured
Embryo, Mammalian
Gene Expression
Mice / immunology
Proto-Oncogene Proteins / biosynthesis*
Proto-Oncogene Proteins c-bcl-2*
Recombinant Proteins / biosynthesis,  immunology
Stem Cells / immunology
T-Lymphocyte Subsets / cytology,  immunology*
T-Lymphocytes / cytology,  immunology*
bcl-X Protein
Grant Support
ID/Acronym/Agency:
AI-20047/AI/NIAID NIH HHS; AI-35294/AI/NIAID NIH HHS; CA-42335/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD4; 0/Antigens, CD8; 0/Bcl2l1 protein, mouse; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Recombinant Proteins; 0/bcl-X Protein
Comments/Corrections

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