| Bcl-x(l) Bax interaction after transient global ischemia. | |
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MedLine Citation:
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PMID: 9701349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Five minutes of bilateral common carotid artery occlusion in the Mongolian gerbil results in a selective, delayed death of CA1 pyramidal neurons. Although Bcl-2 appears to protect a variety of cells from cell death, the precise role of this apoptosis-regulating protein is complicated. We used immunoblots to estimate levels of Bcl-2, Bcl-x(l), and Bax at various times after carotid occlusion. Rather than Bcl-2, Bcl-x(l) appears to be the predominant neuroprotective form of this family of proto-oncogenes in the gerbil hippocampus. After transient ischemia, Bcl-2 and Bcl-x(l) protein levels remained the same. However, Bax levels were dramatically increased at 6 hours after ischemia, compared with sham-operated animals, and were still elevated at 72 hours after ischemia. To monitor dimerization interactions among theses apoptosis-regulating molecules, immunoprecipitation studies were conducted. These studies demonstrated that Bcl-x(l) association with Bax increases after ischemia. Therefore, Bax may disrupt the more favorable Bcl-x(l) (Bcl-2) interactions necessary for normal neuronal functioning and thus promote transient ischemic death. |
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Authors:
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F J Antonawich; S Krajewski; J C Reed; J N Davis |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Volume: 18 ISSN: 0271-678X ISO Abbreviation: J. Cereb. Blood Flow Metab. Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1998-08-21 Completed Date: 1998-08-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8112566 Medline TA: J Cereb Blood Flow Metab Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 882-6 Citation Subset: IM |
Affiliation:
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Department of Neurology and Program in Neurobiology, State University of New York at Stony Brook, 11794-8121, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis Gerbillinae Hippocampus / metabolism*, pathology* Ischemic Attack, Transient / metabolism*, pathology Male Mice Mice, Inbred C57BL Neuroprotective Agents Proto-Oncogene Proteins / metabolism* Proto-Oncogene Proteins c-bcl-2 / metabolism* Time Factors bcl-2-Associated X Protein bcl-X Protein |
| Grant Support | |
ID/Acronym/Agency:
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NS 30559/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bax protein, mouse; 0/Bcl2l1 protein, mouse; 0/Neuroprotective Agents; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 0/bcl-X Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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