Document Detail


Bcl-X(L) inhibits apoptosis and necrosis produced by Alzheimer's beta-amyloid1-40 peptide in PC12 cells.
MedLine Citation:
PMID:  10507529     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent studies have shown that neuronal apoptosis induced by the Alzheimer's disease (AD) beta-amyloid peptide (Abeta) is related to alteration of the Bax/Bcl-2 ratio. It has been demonstrated that Bcl-X(L) (Bcl-X(L) = protein, bcl-X(L) = gene), a Bcl-2-related protein, prevents apoptosis in mammalian cells. Additionally, TGF-beta1 is able to protect cultured neuronal cells from Abeta-induced apoptosis via upregulation of bcl-X(L) and bcl-2 gene expression. We show that Abeta treatment (500 nM, freshly solubilized) results in apoptosis and necrosis in differentiated PC12 cells maintained with a low dose of NGF-beta (1 ng/ml). To investigate whether transfection of PC12 cells with bcl-X(L) could block Abeta-induced apoptosis, we transfected these cells with a bcl-X(L) construct (pcDNA-bcl-X(L)). Data show that bcl-X(L) significantly inhibits both early-stage apoptosis and late-stage apoptosis/necrosis produced by Abeta treatment (1000 nM) in pcDNA3-bcl-X(L)-transfected PC12 cells as compared with pcDNA3 vector-transfected PC12 cells. These results suggest that Bcl-X(L) exhibits both anti-necrotic as well as anti-apoptotic roles in Abeta-challenged PC12 cells.
Authors:
J Tan; T Town; A Placzek; A Kundtz; H Yu; M Mullan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  272     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-11-09     Completed Date:  1999-11-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  5-8     Citation Subset:  IM    
Affiliation:
The Roskamp Institute, Tampa, FL 33613-4799, USA. jtan@com1.med.usf.edu
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MeSH Terms
Descriptor/Qualifier:
Alzheimer Disease / genetics,  physiopathology
Amyloid beta-Protein / genetics,  pharmacology*,  physiology
Animals
Apoptosis / genetics,  physiology*
Necrosis
Nerve Growth Factor / genetics,  pharmacology
PC12 Cells
Peptide Fragments / genetics,  pharmacology*,  physiology
Proto-Oncogene Proteins c-bcl-2 / genetics,  physiology*
Rats
Transfection / genetics
bcl-X Protein
Chemical
Reg. No./Substance:
0/Amyloid beta-Protein; 0/Bcl2l1 protein, rat; 0/Peptide Fragments; 0/Proto-Oncogene Proteins c-bcl-2; 0/amyloid beta-protein (1-40); 0/bcl-X Protein; 9061-61-4/Nerve Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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