Document Detail

Bcl-2 overexpression disrupts the morphology of PC12 cells through reduced ERK activation.
MedLine Citation:
PMID:  16914120     Owner:  NLM     Status:  MEDLINE    
Bcl-2 has been hypothesized to regulate many cellular functions in addition to its well-characterized role in the prevention of programmed cell death. To understand the role of Bcl-2 in regulating cell morphology and to explore the mechanism of this effect, we examined the effects of Bcl-2 overexpression on the morphology of PC12 cells in culture. We demonstrate that the overexpression of Bcl-2 in PC12 cells results in altered cell morphology and reduced actin expression. Analysis of extracellular signal-regulated kinase (ERK) 1/2 phosphorylation reveals that the morphological changes seen after bcl-2 transfection are associated with reduced ERK activation. Treatment of control (mock-transfected) PC12 cells with the mitogen-activated ERK-activating kinase (MEK) inhibitor PD98059 converts their flat, process-bearing morphology into the rounded, process-free morphology of bcl-2-transfected cells, further confirming the association of ERK activation with altered cell shape. In conclusion, the present study describes a novel function of Bcl-2 in regulating cell shape through reduced ERK activation.
Zhiping Mi; Zeljka Korade Mirnics; Nina Felice Schor
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2006-08-17
Journal Detail:
Title:  Brain research     Volume:  1112     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-18     Completed Date:  2006-11-28     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  46-55     Citation Subset:  IM    
Pediatric Center for Neuroscience, Children's Hospital of Pittsburgh of UPMC, Pittsburgh, PA 15213, USA.
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MeSH Terms
Actins / metabolism
Blotting, Western / methods
Enzyme Activation / physiology
Enzyme Inhibitors / pharmacology
Extracellular Signal-Regulated MAP Kinases / metabolism*
Flavonoids / pharmacology
Gene Expression / physiology*
PC12 Cells / cytology*,  metabolism*
Phalloidine / diagnostic use
Proto-Oncogene Proteins c-bcl-2 / physiology*
Time Factors
Grant Support
Reg. No./Substance:
0/2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; 0/Actins; 0/Enzyme Inhibitors; 0/Flavonoids; 0/Proto-Oncogene Proteins c-bcl-2; 17466-45-4/Phalloidine; EC Signal-Regulated MAP Kinases

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