| Bcl-2 modulates Fas-mediated apoptosis in human renal cell carcinoma cell lines. | |
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MedLine Citation:
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PMID: 11351249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to characterize the role of Bcl-2 expression in Fas-mediated apoptosis in human renal cell carcinoma (RCC) cell lines. RT-PCR analyses showed that 10 different RCC cell lines expressed Fas, but not Fas ligand. Seven of 10 cell lines expressed Fas strongly, while 3 cell lines weakly expressed Fas by flow cytometric analyses. Measurement of the LDH concentration in the culture supernatant revealed that 6 of the 7 cell lines which expressed Fas strongly were sensitive to treatment with an agonistic anti-Fas monoclonal antibody (CH11), whereas all cell lines which weakly expressed Fas did not show Fas-mediated cell death. Furthermore, Bcl-2 expression was found only in three cell lines which were all susceptible to CH11, and the downregulation of Bcl-2 protein by treatment with antisense oligodeoxynucleotide targeting Bcl-2 gene resulted in an enhancement of cell death induced by CH11. These findings suggest that strong Fas expression is necessary for Fas-mediated cell death in RCC cell lines, and Bcl-2 has a protective role against treatment with an anti-Fas monoclonal antibody, despite the fact that Bcl-2 expression was observed only in sensitive cell lines to Fas-mediated cell death. |
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Authors:
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I Hara; S Hara; H Miyake; S Arakawa; S Kamidono |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of oncology Volume: 18 ISSN: 1019-6439 ISO Abbreviation: Int. J. Oncol. Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-05-14 Completed Date: 2001-07-26 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: Greece |
Other Details:
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Languages: eng Pagination: 1181-5 Citation Subset: IM |
Affiliation:
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Department of Urology, Kobe University School of Medicine, Kobe 650-0017, Japan. hara@med.kobe-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal Antigen-Antibody Reactions Antigens, CD95 / genetics, immunology, physiology* Apoptosis* Blotting, Western Carcinoma, Renal Cell / metabolism*, pathology Cell Division DNA Fragmentation DNA Primers / chemistry Flow Cytometry Humans Kidney Neoplasms / metabolism*, pathology Oligoribonucleotides, Antisense / pharmacology Proto-Oncogene Proteins / metabolism Proto-Oncogene Proteins c-bcl-2 / metabolism* Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Transfection Tumor Cells, Cultured bcl-2-Associated X Protein |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, CD95; 0/DNA Primers; 0/Oligoribonucleotides, Antisense; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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