Document Detail


Bcl-2 modulates Fas-mediated apoptosis in human renal cell carcinoma cell lines.
MedLine Citation:
PMID:  11351249     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to characterize the role of Bcl-2 expression in Fas-mediated apoptosis in human renal cell carcinoma (RCC) cell lines. RT-PCR analyses showed that 10 different RCC cell lines expressed Fas, but not Fas ligand. Seven of 10 cell lines expressed Fas strongly, while 3 cell lines weakly expressed Fas by flow cytometric analyses. Measurement of the LDH concentration in the culture supernatant revealed that 6 of the 7 cell lines which expressed Fas strongly were sensitive to treatment with an agonistic anti-Fas monoclonal antibody (CH11), whereas all cell lines which weakly expressed Fas did not show Fas-mediated cell death. Furthermore, Bcl-2 expression was found only in three cell lines which were all susceptible to CH11, and the downregulation of Bcl-2 protein by treatment with antisense oligodeoxynucleotide targeting Bcl-2 gene resulted in an enhancement of cell death induced by CH11. These findings suggest that strong Fas expression is necessary for Fas-mediated cell death in RCC cell lines, and Bcl-2 has a protective role against treatment with an anti-Fas monoclonal antibody, despite the fact that Bcl-2 expression was observed only in sensitive cell lines to Fas-mediated cell death.
Authors:
I Hara; S Hara; H Miyake; S Arakawa; S Kamidono
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of oncology     Volume:  18     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-05-14     Completed Date:  2001-07-26     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1181-5     Citation Subset:  IM    
Affiliation:
Department of Urology, Kobe University School of Medicine, Kobe 650-0017, Japan. hara@med.kobe-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal
Antigen-Antibody Reactions
Antigens, CD95 / genetics,  immunology,  physiology*
Apoptosis*
Blotting, Western
Carcinoma, Renal Cell / metabolism*,  pathology
Cell Division
DNA Fragmentation
DNA Primers / chemistry
Flow Cytometry
Humans
Kidney Neoplasms / metabolism*,  pathology
Oligoribonucleotides, Antisense / pharmacology
Proto-Oncogene Proteins / metabolism
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Transfection
Tumor Cells, Cultured
bcl-2-Associated X Protein
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD95; 0/DNA Primers; 0/Oligoribonucleotides, Antisense; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein

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