Document Detail

Bcl-2 down modulation in WEHI-3B/CTRES cells resistant to Cholera Toxin (CT)-induced apoptosis.
MedLine Citation:
PMID:  16541129     Owner:  NLM     Status:  MEDLINE    
The very different effects of Cholera Toxin (CT) on cell growth and proliferation may depend on the type of ganglioside receptors in cell membranes and different signal transduction mechanisms triggered, but other functions related to the drug resistance mechanisms can not be excluded. The effect of CT treatment on the "in vitro" clonogenicity, the Population Doubling Time (PDT), apoptosis, PKA activation and Bax and Bcl-2 expression was evaluated in WEHI-3B cell line and its CT-resistant subclone (WEHI-3B/CTRES). In WEHI-3B parental cells the dramatic accumulation of cAMP induced by CT correlated well with PKA activation, increased PDT value, inhibition of clonogenicity and apoptosis. H-89 treatment inhibited PKA activation by CT but did not protect the cells from apoptosis and growth inhibition. In WEHI-3B/CTRES no significant CT-dependent accumulation of cAMP occurred with any increase of PKA activity and PDT. In CT resistant cells (WEHI-3B/CTRES), Bcl-2 expression was down regulated by both CT or drug treatment (eg., ciprofloxacin, CPX) although these cells were protected from CT-dependent apoptosis but not from drug-induced apoptosis. Differently from other cell models described, down regulation of Bcl-2 is proved to be independent on cAMP accumulation and PKA activation. Our observations support the implication of cAMP dependent kinase (PKA) in the inhibition of WEHI-3B cells growth and suggest that, in WEHI-3B/CTRES, Bcl-2 expression could be modulated by CT in the absence of cAMP accumulation. Also in consideration of many contradictory data reported in literature, our cell models (of one sensitive parental cell strain and two clones with different uncrossed specific resistance to CT and CPX) provides a new and interesting tool for better investigating the relationship between the CT signal transduction mechanisms and Bcl-2 expression and function.
Augusto Pessina; Cristina Croera; Nicoletta Savalli; Arianna Bonomi; Loredana Cavicchini; Elisa Turlizzi; Fabiana Guizzardi; Lucia Guido; Laura Daprai; Maria Grazia Neri
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell research     Volume:  16     ISSN:  1001-0602     ISO Abbreviation:  Cell Res.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-16     Completed Date:  2006-06-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9425763     Medline TA:  Cell Res     Country:  China    
Other Details:
Languages:  eng     Pagination:  306-12     Citation Subset:  IM    
Institute of Microbiology, University of Milan, Via Pascal 36, 20133 Milan, Italy.
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MeSH Terms
Apoptosis / drug effects*
Cell Line, Tumor
Cell Proliferation / drug effects
Cholera Toxin / pharmacology*
Clone Cells / drug effects
Cyclic AMP-Dependent Protein Kinases / metabolism
Drug Resistance, Neoplasm
Enzyme Activation
Genes, bcl-2 / genetics*
Leukemia, Myeloid
Proto-Oncogene Proteins c-bcl-2 / biosynthesis
bcl-2-Associated X Protein / biosynthesis
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 9012-63-9/Cholera Toxin; EC AMP-Dependent Protein Kinases

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