Document Detail

Bcl-2 and caspase-8 related anoikis resistance in human osteosarcoma MG-63 cells.
MedLine Citation:
PMID:  18675369     Owner:  NLM     Status:  MEDLINE    
Detachment of adherent cells from extracellular matrix results in apoptosis, a process termed "anoikis". Resistance to anoikis is implicated in the progression of many malignancies by facilitating the migration and eventual colonization of distant sites. Human kidney epithelial cells 293T, human osteoblast cells hFOB 1.19 and human osteosarcoma cells Saos-2 significantly underwent anoikis when adherence was prevented. But human osteosarcoma MG-63 cells were distinctly anoikis resistant when detached. They formed large aggregates and showed little apoptosis compared to the other cells. When MG-63 cells were in suspension, caspase-8, physically associated with death receptor was activated by cell-matrix detachment, whereas. Caspase-3 and caspase-9 were not activated. Translational level of Bcl-2 significantly increased in a time-dependent manner, but the level of beta-catenin and PI3K did not. Caspase-8 participates in an anoikis-inducing process in MG-63 cells at an early time, and overexpression of Bcl-2 blocks activation of caspase-8 making MG-63 cells anoikis resistant.
Dingsheng Lin; Jie Feng; Weishan Chen
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Publication Detail:
Type:  Journal Article     Date:  2008-07-15
Journal Detail:
Title:  Cell biology international     Volume:  32     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-20     Completed Date:  2008-12-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  1199-206     Citation Subset:  IM    
Department of Orthopedics, Institute of Orthopaedic Research, 2nd Affiliated Hospital, Medical College, Zhejiang University, #88 Jiefang Road, Hangzhou 310009, PR China.
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MeSH Terms
1-Phosphatidylinositol 3-Kinase / metabolism
Anoikis / physiology*
Caspase 3 / metabolism
Caspase 8 / metabolism*
Caspase 9 / metabolism
Cell Line, Tumor / cytology,  physiology*
Cell Shape
Enzyme Activation
Osteosarcoma / metabolism*
Polyhydroxyethyl Methacrylate / metabolism
Proto-Oncogene Proteins c-bcl-2 / metabolism*
beta Catenin / metabolism
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2; 0/beta Catenin; 25249-16-5/Polyhydroxyethyl Methacrylate; EC 3-Kinase; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9

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