| Bax dimerizes via a symmetric BH3:groove interface during apoptosis. | |
| | |
MedLine Citation:
|
PMID: 22015607 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
During apoptotic cell death, Bax and Bak change conformation and homo-oligomerize to permeabilize mitochondria. We recently reported that Bak homodimerizes via an interaction between the BH3 domain and hydrophobic surface groove, that this BH3:groove interaction is symmetric, and that symmetric dimers can be linked via the α6-helices to form the high order oligomers thought responsible for pore formation. We now show that Bax also dimerizes via a BH3:groove interaction after apoptotic signaling in cells and in mitochondrial fractions. BH3:groove dimers of Bax were symmetric as dimers but not higher order oligomers could be linked by cysteine residues placed in both the BH3 and groove. The BH3:groove interaction was evident in the majority of mitochondrial Bax after apoptotic signaling, and correlated strongly with cytochrome c release, supporting its central role in Bax function. A second interface between the Bax α6-helices was implicated by cysteine linkage studies, and could link dimers to higher order oligomers. We also found that a population of Bax:Bak heterodimers generated during apoptosis formed via a BH3:groove interaction, further demonstrating that Bax and Bak oligomerize via similar mechanisms. These findings highlight the importance of BH3:groove interactions in apoptosis regulation by the Bcl-2 protein family.Cell Death and Differentiation advance online publication, 21 October 2011; doi:10.1038/cdd.2011.138. |
| | |
Authors:
|
G Dewson; S Ma; P Frederick; C Hockings; I Tan; T Kratina; R M Kluck |
Related Documents
:
|
20956147 - Pharmacological actions of statins: potential utility in copd. 17548647 - Cd8+ t cells are required for inflammation and destruction in cigarette smoke-induced e... 19386787 - Cystic fibrosis transmembrane conductance regulator and caveolin-1 regulate epithelial ... 15611267 - Morphine impairs host innate immune response and increases susceptibility to streptococ... 20956857 - Role of various cytokines and growth factors in pubertal development. 15972697 - Novel protein and poxvirus-based vaccine combinations for simultaneous induction of hum... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-10-21 |
Journal Detail:
|
Title: Cell death and differentiation Volume: - ISSN: 1476-5403 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-10-21 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9437445 Medline TA: Cell Death Differ Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia [2] Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Genetically defining the mechanism of Puma- and Bim-induced apoptosis.
Next Document: SCaMC-1 promotes cancer cell survival by desensitizing mitochondrial permeability transition via ATP...