Document Detail

Basophil sensitivity in patients not responding to venom immunotherapy.
MedLine Citation:
PMID:  18270492     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Treatment failure of venom immunotherapy (VIT) is not rare and the risk and pathogenic factors for those failures are so far poorly understood. For that reason we evaluated allergen-specific basophil sensitivity in patients who did not tolerate field re-stings after completed VIT treatment. METHODS: Basophil responsiveness was evaluated by flow cytometry analyses of basophil CD63 surface expression induced by different concentrations of bee or wasp venom (1, 0.1 and 0.01 microg/ml) in 14 treated patients who had experienced systemic allergic reactions (Muller grades II-III) and 17 treated patients who had no reactions after the field re-stings. We also included a group of 28 Hymenoptera venom-allergic patients who had not received VIT. RESULTS: In 14 patients who still reacted to bee or wasp sting, basophil response at a venom concentration of 0.1 microg/ml was significantly higher than in patients who tolerated field re-stings (p = 0.03; t test). Basophil response was also slightly higher at a concentration of 1 microg/ml, but not to statistical significance (p = 0.12; t test). There was no difference in the response to direct cross-linking of the IgE and in venom-specific IgE and IgG4 serum concentrations between those 2 groups (p > 0.8; Fisher's exact test, t test). Patients who tolerated field re-stings have also significantly lower basophil response in comparison to patients who had not received VIT, both at 0.1 and 1 microg/ml of venom concentrations (p < 0.001; t test). CONCLUSIONS: The results suggest that basophil venom-specific sensitivity is associated with the efficiency of VIT.
Andreja Peternelj; Mira Silar; Renato Erzen; Mitja Kosnik; Peter Korosec
Publication Detail:
Type:  Journal Article     Date:  2008-02-13
Journal Detail:
Title:  International archives of allergy and immunology     Volume:  146     ISSN:  1423-0097     ISO Abbreviation:  Int. Arch. Allergy Immunol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-06-13     Completed Date:  2008-07-08     Revised Date:  2010-10-20    
Medline Journal Info:
Nlm Unique ID:  9211652     Medline TA:  Int Arch Allergy Immunol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  248-54     Citation Subset:  IM    
Copyright Information:
(c) 2008 S. Karger AG, Basel
University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia.
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MeSH Terms
Antigens, CD / blood,  immunology*
Arthropod Venoms / immunology,  therapeutic use*
Basophils / immunology*
Desensitization, Immunologic / methods*
Flow Cytometry
Hymenoptera / immunology
Immunoglobulin E / blood,  immunology
Insect Bites and Stings / immunology*,  therapy*
Middle Aged
Platelet Membrane Glycoproteins / immunology*
Statistics, Nonparametric
Reg. No./Substance:
0/Antigens, CD; 0/Arthropod Venoms; 0/CD63 antigen; 0/Platelet Membrane Glycoproteins; 37341-29-0/Immunoglobulin E

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