Document Detail


Basophil activation marker CD203c is useful in the diagnosis of hen's egg and cow's milk allergies in children.
MedLine Citation:
PMID:  20523064     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The diagnosis of food allergy (FA) is usually based on oral food challenge tests (OFC). However, OFCs occasionally induce severe adverse reactions. CD203c expression on basophils is emerging as a potential diagnostic index. We evaluated whether CD203c expression on basophils would be a useful marker of OFC-associated symptoms in hen's egg and cow's milk allergies in children. METHODS: Seventy-one patients who had been diagnosed with FA based on OFCs or a convincing history of FA symptoms in the Department of Pediatrics, Sagamihara National Hospital, were recruited. CD203c expression was assessed after stimulation with antigens (egg white, ovomucoid, milk or casein) using allergenicity kits. The CD203c stimulation index (SI = the allergen-induced CD203c expression level divided by the baseline expression level) and the threshold of CD203c expression (the minimum concentration of antigen to induce CD203c SI >or=2) were analyzed in association with tolerance acquisition. RESULTS: For the CD203c SI, the areas under the receiver-operating characteristic curve were 0.72 for egg white, 0.82 for ovomucoid, 0.84 for milk and 0.67 for casein. The positive predictive value for the threshold of CD203c expression was 94.7% for egg white, 100% for ovomucoid, 85.7% for milk and 75.0% for casein. CONCLUSIONS: Assessment of food antigen-induced CD203c expression on basophils is useful to determine if children will outgrow FA as well as in decision making regarding whether or not to perform OFCs.
Authors:
Sakura Sato; Hiroshi Tachimoto; Akinori Shukuya; Norimasa Kurosaka; Noriyuki Yanagida; Tomohiro Utsunomiya; Masamichi Iguchi; Takatsugu Komata; Takanori Imai; Morimitsu Tomikawa; Motohiro Ebisawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-04
Journal Detail:
Title:  International archives of allergy and immunology     Volume:  152 Suppl 1     ISSN:  1423-0097     ISO Abbreviation:  Int. Arch. Allergy Immunol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-06-04     Completed Date:  2010-07-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9211652     Medline TA:  Int Arch Allergy Immunol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  54-61     Citation Subset:  IM    
Copyright Information:
(c) 2010 S. Karger AG, Basel.
Affiliation:
Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Sagamihara, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Area Under Curve
Basophils / immunology,  metabolism*
Caseins / immunology
Cattle
Child
Child, Preschool
Egg Hypersensitivity / diagnosis*,  immunology
Egg White
Female
Humans
Immunoglobulin E / blood,  immunology
Male
Milk / immunology
Milk Hypersensitivity / diagnosis*,  immunology
Ovomucin / immunology
Phosphoric Diester Hydrolases / analysis*,  immunology,  metabolism
Predictive Value of Tests
Pyrophosphatases / analysis*,  immunology,  metabolism
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Caseins; 0/ENPP3 protein, human; 37281-36-0/Ovomucin; 37341-29-0/Immunoglobulin E; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 3.6.1.-/Pyrophosphatases

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