Document Detail


Basidiospore allergens: determination of optimal extraction methods.
MedLine Citation:
PMID:  3608137     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Five methods were tested by RAST for allergen extraction from seven basidiospore species Armillaria tabescens, Chlorophyllum molybdites, Coprinus quadrifidus, Pleurotus ostreatus, Calvatia cyathiformis, Pisolithus tinctorius, and Scleroderma sp. With each basidiospore type, extracted allergen activity varied according to the method employed. In general, defatting of spores with ethyl ether, followed by homogenization in 0.125 M NH4HCO3 buffer, resulted in greatest allergen yield. In order to compare extracts from different isolates, batches of Pleurotus ostreatus spores obtained from different locations and over different time periods were analysed. Spores harvested from the same basidiomycete species in different areas varied in allergen (RAST) and protein (HPLC profile) content. The spores obtained from the same location over a 1-2 year period did not differ significantly. These results indicate that basidiospores can be stored for several years and that spore extracts from different locations can vary. These studies will help in the future to provide better characterized extracts for clinical studies.
Authors:
V Liengswangwong; J E Salvaggio; F L Lyon; S B Lehrer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical allergy     Volume:  17     ISSN:  0009-9090     ISO Abbreviation:  Clin. Allergy     Publication Date:  1987 May 
Date Detail:
Created Date:  1987-08-31     Completed Date:  1987-08-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0311172     Medline TA:  Clin Allergy     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  191-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Allergens / immunology*
Basidiomycota / immunology*
Chromatography, High Pressure Liquid
Coprinus / immunology
Humans
Methods
Radioallergosorbent Test
Grant Support
ID/Acronym/Agency:
AI-20331/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Allergens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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