| Basic studies on 5-(7-hydroxy-3-O-phosphonocholyl)aminosalicylic acid for the evaluation of microbial overgrowth. | |
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MedLine Citation:
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PMID: 9145212 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A newly synthesized conjugate of 7-hydroxy-3-O-phosphonocholic acid (ursodeoxycholic acid monophosphate) with 5-aminosalicylic acid (5-ASA-UDCA monophosphate) was investigated to determine its suitability for the evaluation of enteric bacteria. This compound, 5-ASA-UDCA monophosphate, was efficiently deconjugated by cholylglycine hydrolase to release 5-ASA, whereas it was completely resistant to deconjugation by pancreatic and intestinal mucosal enzymes. In everted gut sac experiments, 5-ASA-UDCA monophosphate was not actively absorbed from any part of the small intestine. In animal experiments, urinary excretions of N-acetyl-5-ASA (Ac-5ASA) were measured for 24 h following the oral administration of 20 mg of 5-ASA-UDCA monophosphate. Control rats excreted 276.3 +/- 89.0 micrograms (mean +/- S.E.) of Ac-5ASA, whereas the rats with intestinal bacterial overgrowth excreted more (1224.1 +/- 231.5 micrograms; p < 0.01). These basic studies indicate that this compound is likely to offer a simple method for the evaluation of microbial overgrowth without the use of radioisotopes or expensive, special apparatus. |
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Authors:
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T Konishi; M Takahashi; S Ohta |
Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 20 ISSN: 0918-6158 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 1997 Apr |
Date Detail:
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Created Date: 1997-07-03 Completed Date: 1997-07-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: JAPAN |
Other Details:
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Languages: eng Pagination: 370-5 Citation Subset: IM |
Affiliation:
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Department of Hospital Pharmacy, Chugoku Rosai Hospital, Hiroshima, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aminosalicylic Acids
/
chemical synthesis,
diagnostic use*,
pharmacokinetics Animals Bacteria / growth & development* Bile / chemistry Humans Intestines / metabolism, microbiology* Liver / enzymology Male Pancreatin / metabolism Rats Rats, Wistar Ursodeoxycholic Acid / analogs & derivatives*, chemical synthesis, diagnostic use, pharmacokinetics |
| Chemical | |
Reg. No./Substance:
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0/5-(7-hydroxy-3-O-phosphonocholyl)aminosalicylic acid; 0/Aminosalicylic Acids; 128-13-2/Ursodeoxycholic Acid; 8049-47-6/Pancreatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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