Document Detail

Basic properties of the pyruvate dehydrogenase complex isolated from aurochs heart.
MedLine Citation:
PMID:  7732764     Owner:  NLM     Status:  MEDLINE    
The purified aurochs (Bison bonasus, European bison) heart pyruvate dehydrogenase complex (PDC) has a set of subunits typical of mammalian PDC. PDC from aurochs heart contains firmly bound tiamine pyrophosphate in the amount providing over 50% of the maximal activity of the complex. The apparent value for activation energy of PDC is 60 kJ/mol. The Michaelis constant values for aurochs heart PDC are 22.4 +/- 1.0, 3.3 +/- 0.1 and 24.4 +/- 3.6 microM for pyruvate, CoA and NAD, accordingly. Acetyl-CoA is a competitive inhibitor with respect to CoA (Ki = 14.2 +/- 0.4 microM), whereas NADH gives the same inhibition with respect to NAD (Ki = 46.9 +/- 10.0 microM). The Km for CoA and NAD of the aurochs heart PDC are lower than that of domestic animals PDC.
M Czygier; S A Strumiło
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta biochimica Polonica     Volume:  41     ISSN:  0001-527X     ISO Abbreviation:  Acta Biochim. Pol.     Publication Date:  1994  
Date Detail:
Created Date:  1995-05-30     Completed Date:  1995-05-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  14520300R     Medline TA:  Acta Biochim Pol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  453-7     Citation Subset:  IM    
Department of Biochemistry, University of Warsaw, Białystok, Poland.
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MeSH Terms
Myocardium / enzymology*
Pyruvate Dehydrogenase Complex / chemistry*,  isolation & purification*
Reg. No./Substance:
0/Pyruvate Dehydrogenase Complex

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