Document Detail

Basic fibroblast growth factor-induced decrease in type I collagen gene transcription is mediated by B-myb.
MedLine Citation:
PMID:  9751116     Owner:  NLM     Status:  MEDLINE    
Basic fibroblast growth factor (bFGF), a member of the fibroblast growth factor family, potently induces increased vascular smooth muscle cell (SMC) proliferation and decreased expression of type I collagen. Recently, our laboratory demonstrated that, in bovine vascular SMCs, expression of B-myb, a member of the myb gene family, is dependent upon cellular growth state and that B-myb decreases alpha1(I) collagen promoter activity in transient transfection assays. Nuclear run-off analysis indicated that the decrease in alpha1(I) collagen mRNA level seen upon bFGF treatment was due to a decline in the rate of alpha1(I) procollagen gene transcription. Thus, we investigated the potential role of B-Myb in the down-regulation of type I collagen gene expression by bFGF. Using Northern blot analysis, we found that bFGF treatment of bovine aortic SMCs caused an increase in B-myb mRNA levels. Ectopic expression of B-myb decreased endogenous alpha1(I) collagen mRNA levels. Importantly, introduction of a B-myb antisense oligonucleotide prevented the drop in the alpha1(I) collagen mRNA levels seen upon treatment with bFGF. Together, these results indicate that B-myb mediates signals leading to the decreased rate of alpha1(I) collagen gene transcription caused by bFGF.
K E Kypreos; M A Nugent; G E Sonenshein
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research     Volume:  9     ISSN:  1044-9523     ISO Abbreviation:  Cell Growth Differ.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-12-11     Completed Date:  1998-12-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9100024     Medline TA:  Cell Growth Differ     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  723-30     Citation Subset:  IM    
Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118, USA.
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MeSH Terms
Cell Cycle Proteins*
Cells, Cultured
Collagen / drug effects,  genetics*
DNA-Binding Proteins / drug effects,  genetics,  physiology*
Dose-Response Relationship, Drug
Fibroblast Growth Factor 2 / pharmacology*
Gene Expression Regulation / drug effects
Genes / drug effects,  genetics*
Oligonucleotides, Antisense / pharmacology
Procollagen / drug effects,  genetics
RNA, Messenger / drug effects,  metabolism
Trans-Activators / drug effects,  genetics,  physiology*
Transcription, Genetic / drug effects
Grant Support
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/Oligonucleotides, Antisense; 0/Procollagen; 0/RNA, Messenger; 0/Trans-Activators; 103107-01-3/Fibroblast Growth Factor 2; 9007-34-5/Collagen

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