Document Detail


Basic fibroblast growth factor accumulates in the nuclei of various bFGF-producing cell types.
MedLine Citation:
PMID:  2246329     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The intracellular localization of basic fibroblast growth factor (bFGF) was studied in BHK-21 cells transfected with an expression vector containing the complementary DNA (cDNA) of the human bFGF gene (pbFGF). The intracellular location of bFGF was determined using indirect immunofluorescence. The antibodies used were polyclonal antibodies directed against either recombinant human bFGF or recombinant Xenopus bFGF. The nuclei of transfected cells that produce bFGF, but not the nuclei of untransfected cells, were labeled strongly by the antibodies. The nuclear staining was totally abolished when anti-bFGF antibodies preadsorbed with bFGF were used. Several types of endothelial cells known to produce bFGF were also stained in their nuclei by the antibodies. Nuclear extracts prepared from transfected cells were found to contain bFGF as determined using heparin-sepharose affinity chromatography, followed by Western blot analysis of fractions, which stimulated the proliferation BHK-21 cells. The mitogenic activity associated with the nuclei was not destroyed when isolated cell nuclei were digested by trypsin. It is therefore likely that the nucleus associated bFGF is intranuclear. These findings suggest that some biological activities of bFGF may be mediated by nuclear bFGF binding proteins or by the direct binding of bFGF to DNA.
Authors:
S Tessler; G Neufeld
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  145     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-01-08     Completed Date:  1991-01-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  310-7     Citation Subset:  IM    
Affiliation:
Department of Biology, Technion, Israel Institute of Technology, Haifa.
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MeSH Terms
Descriptor/Qualifier:
Adrenal Glands / blood supply
Animals
Aorta / cytology
Blotting, Western
Cattle
Cell Fractionation
Cell Nucleus / metabolism*
Cells, Cultured
Cricetinae
Endothelium, Corneal / cytology
Endothelium, Vascular / cytology
Fibroblast Growth Factor 2 / biosynthesis,  genetics,  metabolism*
Fluorescent Antibody Technique
Humans
Middle Aged
Plasmids
Transfection
Chemical
Reg. No./Substance:
103107-01-3/Fibroblast Growth Factor 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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