| Basic control of reperfusion effectively protects against reperfusion injury in a realistic rodent model of acute limb ischemia. | |
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MedLine Citation:
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PMID: 18936330 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Reperfusion injury is insufficiently addressed in current clinical management of acute limb ischemia. Controlled reperfusion carries an enormous clinical potential and was tested in a new reality-driven rodent model. METHODS AND RESULTS: Acute hind-limb ischemia was induced in Wistar rats and maintained for 4 hours. Unlike previous tourniquets models, femoral vessels were surgically prepared to facilitate controlled reperfusion and to prevent venous stasis. Rats were randomized into an experimental group (n=7), in which limbs were selectively perfused with a cooled isotone heparin solution at a limited flow rate before blood flow was restored, and a conventional group (n=7; uncontrolled blood reperfusion). Rats were killed 4 hours after blood reperfusion. Nonischemic limbs served as controls. Ischemia/reperfusion injury was significant in both groups; total wet-to-dry ratio was 159+/-44% of normal (P=0.016), whereas muscle viability and contraction force were reduced to 65+/-13% (P=0.016) and 45+/-34% (P=0.045), respectively. Controlled reperfusion, however, attenuated reperfusion injury significantly. Tissue edema was less pronounced (132+/-16% versus 185+/-42%; P=0.011) and muscle viability (74+/-11% versus 57+/-9%; P=0.004) and contraction force (68+/-40% versus 26+/-7%; P=0.045) were better preserved than after uncontrolled reperfusion. Moreover, subsequent blood circulation as assessed by laser Doppler recovered completely after controlled reperfusion but stayed durably impaired after uncontrolled reperfusion (P=0.027). CONCLUSIONS: Reperfusion injury was significantly alleviated by basic modifications of the initial reperfusion period in a new in vivo model of acute limb ischemia. With this model, systematic optimizations of according protocols may eventually translate into improved clinical management of acute limb ischemia. |
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Authors:
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Florian Dick; Jianhui Li; Marie-Noëlle Giraud; Christoph Kalka; Juerg Schmidli; Hendrik Tevaearai |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-20 |
Journal Detail:
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Title: Circulation Volume: 118 ISSN: 1524-4539 ISO Abbreviation: Circulation Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-04 Completed Date: 2008-11-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0147763 Medline TA: Circulation Country: United States |
Other Details:
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Languages: eng Pagination: 1920-8 Citation Subset: AIM; IM |
Affiliation:
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Department of Cardiovascular Surgery, Inselspital, and University of Bern, Berne, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acute Disease Animals Anticoagulants / pharmacology Disease Models, Animal* Edema / drug therapy, physiopathology, prevention & control Female Femoral Artery / physiology, surgery, ultrasonography Heparin / pharmacology Hindlimb / blood supply* Laser-Doppler Flowmetry Male Muscle Contraction Peripheral Vascular Diseases / drug therapy, physiopathology, prevention & control Postthrombotic Syndrome / drug therapy, physiopathology, prevention & control Rats Rats, Wistar* Regional Blood Flow Reperfusion Injury / drug therapy, physiopathology*, prevention & control* Tourniquets |
| Chemical | |
Reg. No./Substance:
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0/Anticoagulants; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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