Document Detail

Basic blue 148: a rapid stain for T helper cells.
MedLine Citation:
PMID:  7578596     Owner:  NLM     Status:  MEDLINE    
After brief exposure to an aqueous solution of the oxazine textile dye C.I. basic blue 148 following fixation in 37% formalin, 95% ethanol and glacial acetic acid, T helper cell nuclei and cytoplasm in specimens of peripheral blood displayed a deep red-violet color. No other cell in normal blood or bone marrow specimens showed intense staining of this type. The total staining time is 1 min. Basic blue 148 stain is a promising technique for hematology and immunology laboratories as a rapid screening test for T helper cells in blood specimens using a microscopic slide and ordinary incandescent illumination.
K Kass
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  70     ISSN:  1052-0295     ISO Abbreviation:  Biotech Histochem     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-11-30     Completed Date:  1995-11-30     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  99-104     Citation Subset:  IM    
Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
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MeSH Terms
Coloring Agents*
T-Lymphocytes, Helper-Inducer / cytology*
Reg. No./Substance:
0/C.I. basic blue 148; 0/Coloring Agents; 0/Oxazines

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