Document Detail


Basic blue 148: a rapid stain for T helper cells.
MedLine Citation:
PMID:  7578596     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
After brief exposure to an aqueous solution of the oxazine textile dye C.I. basic blue 148 following fixation in 37% formalin, 95% ethanol and glacial acetic acid, T helper cell nuclei and cytoplasm in specimens of peripheral blood displayed a deep red-violet color. No other cell in normal blood or bone marrow specimens showed intense staining of this type. The total staining time is 1 min. Basic blue 148 stain is a promising technique for hematology and immunology laboratories as a rapid screening test for T helper cells in blood specimens using a microscopic slide and ordinary incandescent illumination.
Authors:
K Kass
Related Documents :
6179426 - Epithelial membrane antigen. its use in the cytodiagnosis of malignancy in serous effus...
6295136 - An immunoperoxidase investigation of s-100 protein in granular cell myoblastomas: evide...
11465476 - Immunoelectron microscopy on material retrieved from paraffin: accurate sampling on the...
60316 - Ultrahistochemical study on the ruthenium red surface staining. ii. nature and affinity...
1448456 - Element distribution in organelles of pancreatic acinar cells of rat, mouse, and pig in...
361426 - Studies by radioiodination of normal adult, fetal and leukemic cell membranes.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  70     ISSN:  1052-0295     ISO Abbreviation:  Biotech Histochem     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-11-30     Completed Date:  1995-11-30     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  99-104     Citation Subset:  IM    
Affiliation:
Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Coloring Agents*
Humans
Oxazines*
T-Lymphocytes, Helper-Inducer / cytology*
Chemical
Reg. No./Substance:
0/C.I. basic blue 148; 0/Coloring Agents; 0/Oxazines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Use of DAPI for anastomosis group typing of strains of the fungus Rhizoctonia solani.
Next Document:  Argyrophilic nucleolar organizer regions and bromodeoxyuridine and 3[H]-thymidine labelling indices ...