Document Detail

Base-pairing interactions involving the 5' and 3'-terminal nucleotides of group II self-splicing introns.
MedLine Citation:
PMID:  2191139     Owner:  NLM     Status:  MEDLINE    
By combining comparative sequence analyses and nucleotide replacements, we show that formation of the active center of group II introns rests in part on two novel long-range base-pairing interactions. (1) The last nucleotide of group II introns forms a solitary Watson-Crick base-pair with one of the nucleotides in the short sequence stretch connecting domains II and III. Formation of this base-pair is rate-limiting for the 3' cleavage and ligation step. (2) Nucleotides 3 and 4 form base-pairs with two consecutive nucleotides in a well-conserved internal loop of domain I. This interaction is involved in both the 5' and 3' cleavage steps. Possible relationships between group II and nuclear pre-mRNA introns are reassessed by taking into account these new pieces of information.
A Jacquier; F Michel
Related Documents :
19789289 - Extensive structural renovation of retrogenes in the evolution of the populus genome.
16819719 - Comparative analyses of the structure of the 1,3-beta-glucan synthase gene in paracocci...
7574489 - Structure and activities of group ii introns.
9344739 - The preferential mode analysis of dna sequence.
20587589 - A possible role for short introns in the acquisition of stroma-targeting peptides in th...
11861899 - Compilation and analysis of group ii intron insertions in bacterial genomes: evidence f...
9820789 - Genomic structure and developmental expression of the chicken nonocarboxylate transport...
9249029 - Investigation of different recombinant isoforms of grass group-v allergens (timothy gra...
8369339 - Analysis of chimeric spinach/cyanobacterial cp43 mutants of synechocystis sp. pcc 6803:...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular biology     Volume:  213     ISSN:  0022-2836     ISO Abbreviation:  J. Mol. Biol.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-07-16     Completed Date:  1990-07-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985088R     Medline TA:  J Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  437-47     Citation Subset:  IM    
Institut Pasteur, Département de Biologie Moléculaire, Paris, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacteriophages / genetics
Base Composition
Base Sequence
Genes, Regulator*
Molecular Sequence Data
Nucleic Acid Conformation
Poly A / genetics*
RNA Caps / genetics*
RNA Splicing*
RNA, Catalytic
RNA, Ribosomal / genetics*
Terminator Regions, Genetic*
Reg. No./Substance:
0/RNA Caps; 0/RNA, Catalytic; 0/RNA, Ribosomal; 24937-83-5/Poly A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Estimating the total number of lymphatic valves in infant lungs with the fractionator.
Next Document:  Translated translational operator in Escherichia coli. Auto-regulation in the infC-rpmI-rplT operon.