Document Detail


Base-pairing interactions involving the 5' and 3'-terminal nucleotides of group II self-splicing introns.
MedLine Citation:
PMID:  2191139     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
By combining comparative sequence analyses and nucleotide replacements, we show that formation of the active center of group II introns rests in part on two novel long-range base-pairing interactions. (1) The last nucleotide of group II introns forms a solitary Watson-Crick base-pair with one of the nucleotides in the short sequence stretch connecting domains II and III. Formation of this base-pair is rate-limiting for the 3' cleavage and ligation step. (2) Nucleotides 3 and 4 form base-pairs with two consecutive nucleotides in a well-conserved internal loop of domain I. This interaction is involved in both the 5' and 3' cleavage steps. Possible relationships between group II and nuclear pre-mRNA introns are reassessed by taking into account these new pieces of information.
Authors:
A Jacquier; F Michel
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular biology     Volume:  213     ISSN:  0022-2836     ISO Abbreviation:  J. Mol. Biol.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-07-16     Completed Date:  1990-07-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985088R     Medline TA:  J Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  437-47     Citation Subset:  IM    
Affiliation:
Institut Pasteur, Département de Biologie Moléculaire, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Bacteriophages / genetics
Base Composition
Base Sequence
Genes, Regulator*
Introns*
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
Poly A / genetics*
RNA Caps / genetics*
RNA Splicing*
RNA, Catalytic
RNA, Ribosomal / genetics*
Terminator Regions, Genetic*
Chemical
Reg. No./Substance:
0/RNA Caps; 0/RNA, Catalytic; 0/RNA, Ribosomal; 24937-83-5/Poly A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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