Document Detail


Base pair mismatch recognition using plasmon resonant particle labels.
MedLine Citation:
PMID:  12381369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We demonstrate the use of silver plasmon resonant particles (PRPs), as reporter labels, in a microarray-based DNA hybridization assay in which we screen for a known polymorphic site in the breast cancer gene BRCA1. PRPs (40-100 nm in diameter) image as diffraction-limited points of colored light in a standard microscope equipped with dark-field illumination, and can be individually identified and discriminated against background scatter. Rather than overall intensity, the number of PRPs counted in a CCD image by a software algorithm serves as the signal in these assays. In a typical PRP hybridization assay, we achieve a detection sensitivity that is approximately 60 x greater than that achieved by using fluorescent labels. We conclude that single particle counting is robust, generally applicable to a wide variety of assay platforms, and can be integrated into low-cost and quantitative detection systems for single nucleotide polymorphism analysis.
Authors:
Steven J Oldenburg; Christine C Genick; Keith A Clark; David A Schultz
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  309     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-10-16     Completed Date:  2003-05-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  109-116     Citation Subset:  IM    
Affiliation:
Seashell Technology, LLC 3252 Holiday Court Suite 227, La Jolla, CA 92037, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Pair Mismatch*
Base Sequence
Biotinylation
Breast Neoplasms / genetics
DNA / chemistry,  genetics*
Female
Genes, BRCA1
Humans
Molecular Sequence Data
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis / methods*
Polymorphism, Single Nucleotide
Sensitivity and Specificity
Surface Plasmon Resonance / instrumentation,  methods*
Templates, Genetic
Grant Support
ID/Acronym/Agency:
1R43AG186940/AG/NIA NIH HHS; 2R42RR12415/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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