Document Detail


Basal release of NO in the mesenteric artery in portal hypertension and cirrhosis: role of dimethylarginine dimethylaminohydrolase.
MedLine Citation:
PMID:  23302093     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND AND AIM: Increased basal release of nitric oxide (NO) in the splanchnic circulation contributes to elevated plasma levels of NO observed in decompensated cirrhosis. We evaluated in rat mesenteric arteries whether the differences in basal release of NO, revealed by asymmetric dimethylarginine (ADMA)- and N(G) -nitro-L-arginine methyl ester (L-NAME)-induced contractions, were associated with changes in mRNA expression of endothelial NO synthase (eNOS) and dimethylarginine dimethylaminohydrolases (DDAHs). METHODS: Rat small mesenteric arteries from 14 Sham-control, from 14 with pre-hepatic portal hypertension (PPVL) and from 14 with bile duct excision (BDE)-induced cirrhosis were precontracted under isometric conditions with norepinephrine and additional contractions were induced with ADMA and L-NAME. mRNA expression of eNOS, DDAH-1 and DDAH-2 in mesenteric arteries were evaluated by real-time PCR. RESULTS: ADMA and L-NAME caused concentration- and endothelium-dependent contractions. pD(2) values to L-NAME were similar in all groups. In contrast, pD(2) values to ADMA were similar in PPVL and BDE but were significantly lower than those of the L-NAME and the Sham groups. Relaxation to acetylcholine was not modified by ADMA or L-NAME but was abolished by charybdotoxin plus apamin. There was an increased mRNA expression of eNOS, DDAH-1 and DDAH-2 in mesenteric arteries from PPVL and BDE compared to the Sham group. CONCLUSION: Basal release of NO is increased in mesenteric arteries of PPVL and BDE rats. The rise in expression of DDAHs indicates a higher degradation of ADMA. This would result in an increased generation of endothelial NO and mesenteric vasodilation.
Authors:
Eva Serna; María Dolores Mauricio; Paloma Lluch; Gloria Segarra; Belén Cortina; Salvador Lluch; Pascual Medina
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-10
Journal Detail:
Title:  Journal of gastroenterology and hepatology     Volume:  -     ISSN:  1440-1746     ISO Abbreviation:  J. Gastroenterol. Hepatol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607909     Medline TA:  J Gastroenterol Hepatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd.
Affiliation:
Departamento de Fisiología, Facultad de Medicina y Odontología, Universidad de Valencia, Av. Blasco Ibáñez 15, 46010, Valencia, Spain; Instituto de Investigación Sanitaria INCLIVA, Hospital Clínico Universitario, 46010, Valencia, Spain.
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