| Basal medium development for serum-free culture: a historical perspective. | |
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MedLine Citation:
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PMID: 22358525 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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The evolution of basal synthetic formulations to support mammalian cell culture applications has been facilitated by the contributions of many investigators. Definition of minimally-required nutrient categories by Harry Eagle in the 1950's spawned an iterative process of continuous modification and refinement of the exogenous environment to cultivate new cell types and to support emerging applications of cultured mammalian cells. Key historical elements are traced, leading to the development of high potency, basal nutrient formulations capable of sustaining serum-free proliferation and biological production. Emerging techniques for alimentation of fed batch and continuous perfusion bioreactors, using partial nutrient concentrates deduced from spent medium analysis, can enhance medium utilization and bioreactor productivity. |
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Authors:
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D Jayme; T Watanabe; T Shimada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytotechnology Volume: 23 ISSN: 0920-9069 ISO Abbreviation: Cytotechnology Publication Date: 1997 Jan |
Date Detail:
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Created Date: 2012-02-23 Completed Date: 2012-10-02 Revised Date: 2013-04-01 |
Medline Journal Info:
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Nlm Unique ID: 8807027 Medline TA: Cytotechnology Country: Netherlands |
Other Details:
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Languages: eng Pagination: 95-101 Citation Subset: - |
Affiliation:
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Life Technologies, Inc., Grand Island, NY, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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