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Basal medium development for serum-free culture: a historical perspective.
MedLine Citation:
PMID:  22358525     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The evolution of basal synthetic formulations to support mammalian cell culture applications has been facilitated by the contributions of many investigators. Definition of minimally-required nutrient categories by Harry Eagle in the 1950's spawned an iterative process of continuous modification and refinement of the exogenous environment to cultivate new cell types and to support emerging applications of cultured mammalian cells. Key historical elements are traced, leading to the development of high potency, basal nutrient formulations capable of sustaining serum-free proliferation and biological production. Emerging techniques for alimentation of fed batch and continuous perfusion bioreactors, using partial nutrient concentrates deduced from spent medium analysis, can enhance medium utilization and bioreactor productivity.
Authors:
D Jayme; T Watanabe; T Shimada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytotechnology     Volume:  23     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  1997 Jan 
Date Detail:
Created Date:  2012-02-23     Completed Date:  2012-10-02     Revised Date:  2013-04-01    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  95-101     Citation Subset:  -    
Affiliation:
Life Technologies, Inc., Grand Island, NY, USA.
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