Document Detail


Basal lamina inhibition suppresses synthesis of calcium-dependent proteins associated with mammary epithelial cell spreading.
MedLine Citation:
PMID:  3720859     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Spreading of mouse mammary epithelial cells on collagen gels is closely correlated with the synthesis of a group of putative calcium-binding proteins (CBP) (Braslau et al., Exp cell res 155 (1984) 213). Collagen synthesis was shown to occur during cell spreading, while omission of serum prevented cell spreading and the synthesis of collagen. The proline analogues cis-hydroxyproline and L-azetidine-2-carboxylic acid were shown to inhibit epithelial cell spreading and to suppress the collagen synthesis that occurs during serum-supported cell spreading. Inhibition of collagen synthesis resulted in the inhibition of CBP synthesis associated with cell spreading. In contrast, the collagen cross-linking inhibitor B-aminopropionitrile did not inhibit cell spreading nor did it suppress collagen synthesis; CBP synthesis was also normal during treatment with this inhibitor. Thus, mammary epithelial cell spreading on collagen gels and CBP synthesis can both be suppressed by inhibition of collagen synthesis indicating that they may be integrated in some manner. It is suggested that inhibition of cell spreading during inhibition of collagen synthesis results from failure to assemble a normal basal lamina; this may in turn signal suppression of CBP synthesis.
Authors:
V Rocha; Y K Hom; M P Marinkovich
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  165     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1986 Aug 
Date Detail:
Created Date:  1986-08-08     Completed Date:  1986-08-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  450-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Basement Membrane / physiology*
Calcium-Binding Proteins / physiology*
Cell Adhesion
Cell Movement
Cells, Cultured
Collagen / physiology*
Epithelial Cells
Extracellular Matrix / physiology*
Female
Mammary Glands, Animal / cytology*
Mice
Grant Support
ID/Acronym/Agency:
RRO8132/RR/NCRR NIH HHS; T34-07910//PHS HHS
Chemical
Reg. No./Substance:
0/Calcium-Binding Proteins; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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