Document Detail

Basal extracellular signal-regulated kinase activity modulates cell-cell and cell-matrix interactions.
MedLine Citation:
PMID:  9584166     Owner:  NLM     Status:  MEDLINE    
Suppression of the basal extracellular signal-regulated kinase (ERK) activity in PC12 cells markedly altered their phenotype. Wild-type cells grew in a dissociated pattern adherent to the substrate. The stable expression of an ERK inhibitory mutant resulted in the formation of calcium-dependent aggregates which were less adherent to the substrate. Concomitantly, the cells reorganized their actin cytoskeleton and increased their expression of several adherens junction proteins, particularly cadherin. Metabolic labeling demonstrated an increased synthesis of cadherin and beta-catenin in these cells. Nontransfected PC12 cells and a ras-transformed MDCK cell line also formed aggregates and increased their expression of adherens junction proteins following treatment with the selective MEK inhibitor PD98059. A peptide containing the HAV cadherin recognition sequence attenuated the aggregation. These studies suggest that in PC12 and epithelial cells, ERKs are pivotally positioned to enhance substrate interactions when active or to release homotypic interactions when suppressed.
Q Lu; M Paredes; J Zhang; K S Kosik
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  18     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-06-17     Completed Date:  1998-06-17     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3257-65     Citation Subset:  IM    
Center for Neurologic Diseases, Brigham and Women's Hospital, Boston, MA 02115, USA.
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MeSH Terms
Actins / metabolism
Antigens, CD
Cadherins / biosynthesis
Calcium / metabolism
Calcium-Calmodulin-Dependent Protein Kinases / antagonists & inhibitors,  genetics,  metabolism,  physiology*
Cell Adhesion Molecules / metabolism
Cell Communication*
Cell Line
Cytoskeletal Proteins / biosynthesis
Enzyme Inhibitors / pharmacology
Extracellular Matrix / physiology*
Flavonoids / pharmacology
Mitogen-Activated Protein Kinase 1
PC12 Cells
beta Catenin
Reg. No./Substance:
0/2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; 0/Actins; 0/Antigens, CD; 0/CDH2 protein, human; 0/Cadherins; 0/Catnb protein, rat; 0/Cell Adhesion Molecules; 0/Cytoskeletal Proteins; 0/Enzyme Inhibitors; 0/Flavonoids; 0/Trans-Activators; 0/beta Catenin; 7440-70-2/Calcium; EC Protein Kinases; EC Protein Kinase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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