Document Detail


Barrier activity in Candida albicans mediates pheromone degradation and promotes mating.
MedLine Citation:
PMID:  17416895     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mating in Candida albicans and Saccharomyces cerevisiae is regulated by the secretion of peptide pheromones that initiate the mating process. An important regulator of pheromone activity in S. cerevisiae is barrier activity, involving an extracellular aspartyl protease encoded by the BAR1 gene that degrades the alpha pheromone. We have characterized an equivalent barrier activity in C. albicans and demonstrate that the loss of C. albicans BAR1 activity results in opaque a cells exhibiting hypersensitivity to alpha pheromone. Hypersensitivity to pheromone is clearly seen in halo assays; in response to alpha pheromone, a lawn of C. albicans Deltabar1 mutant cells produces a marked zone in which cell growth is inhibited, whereas wild-type strains fail to show halo formation. C. albicans mutants lacking BAR1 also exhibit a striking mating defect in a cells, but not in alpha cells, due to overstimulation of the response to alpha pheromone. The block to mating occurs prior to cell fusion, as very few mating zygotes were observed in mixes of Deltabar1 a and alpha cells. Finally, in a barrier assay using a highly pheromone-sensitive strain, we were able to demonstrate that barrier activity in C. albicans is dependent on Bar1p. These studies reveal that a barrier activity to alpha pheromone exists in C. albicans and that the activity is analogous to that caused by Bar1p in S. cerevisiae.
Authors:
Dana Schaefer; Pierre Côte; Malcolm Whiteway; Richard J Bennett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-06
Journal Detail:
Title:  Eukaryotic cell     Volume:  6     ISSN:  1535-9778     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-08     Completed Date:  2007-12-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  907-18     Citation Subset:  IM    
Affiliation:
MMI Department, Brown University, 171 Meeting Street, Providence, RI 02912, USA.
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MeSH Terms
Descriptor/Qualifier:
Aspartic Acid Endopeptidases / genetics,  metabolism
Base Sequence
Candida albicans / cytology,  genetics,  physiology*
Cell Shape
Gene Deletion
Genes, Mating Type, Fungal*
Humans
Molecular Sequence Data
Mutation
Peptides / metabolism*
Pheromones / metabolism*
Receptors, Mating Factor / metabolism
Saccharomyces cerevisiae Proteins / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Peptides; 0/Pheromones; 0/Receptors, Mating Factor; 0/Saccharomyces cerevisiae Proteins; 61194-02-3/mating factor; EC 3.4.23.-/Aspartic Acid Endopeptidases; EC 3.4.23.-/BAR1 protein, S cerevisiae
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