Document Detail


Barley microsatellites: allele variation and mapping.
MedLine Citation:
PMID:  7727762     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microsatellites have developed into a powerful tool for mapping mammalian genomes and first reports about their use in plants have been published. A database search of 228 barley sequences from GenBank and EMBL was made to determine which simple sequence repeat (SSR) motif prevails in barley. Nearly all types of SSRs were found. The (A)n and (T)n SSRs occurred more often than (C)n and (G)n for n > or = 10. Among the dinucleotide repeats, the (CG)n SSRs occurred least often. Trinucleotide repeats did not occur with n > 7 and there is no correlation between the GC content in the trinucleotide motifs and the number of observed SSRs. Analysing 15 different microsatellites with 11 barleys yielded 2.1 alleles per microsatellite. Sequencing 25 putative microsatellites showed that the resolution capacity of high-quality agarose gels was sufficient to determine differences of only three base pairs. Five microsatellites were mapped on three different chromosomes of a barley RFLP map.
Authors:
J Becker; M Heun
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant molecular biology     Volume:  27     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-05-26     Completed Date:  1995-05-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  835-45     Citation Subset:  IM    
Affiliation:
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
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MeSH Terms
Descriptor/Qualifier:
Alleles*
Base Sequence
Chromosome Mapping
DNA Primers
DNA, Plant / genetics
DNA, Satellite / genetics*
Genetic Variation*
Hordeum / genetics*
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Plant; 0/DNA, Satellite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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