Document Detail


Banana (Musa sp.).
MedLine Citation:
PMID:  17033061     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cultivated bananas are vegetatively propagating herbs, which are difficult to breed because of widespread male and female sterility. As a complementary gene transfer method in banana, the described Agrobacterium protocol relies on highly regenerable embryogenic cell cultures. Embryogenic cells are infected and co-cultivated in the presence of acetosyringone with Agrobacterium tumefaciens harboring a binary plasmid vector to obtain a mixed population of transformed and untransformed plant cells. Transformed plant cells are promoted to grow for 2 to 3 mo on a cell colony induction medium containing the antibiotics geneticin or hygromycin as selective agents, while agrobacteria are counterselected by timentin. The whole procedure, including plant regeneration, takes approx 6 mo and results in an average frequency of 25 to 50 independent transgenic plants per plate, which equals 50 mg of embryogenic cells. This method has been applied to a wide range of cultivars and to generate large populations of transgenic colonies and plants for tagging genes and promoters in banana.
Authors:
Juan B Pérez Hernández; Serge Remy; Rony Swennen; László Sági
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  344     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-10-11     Completed Date:  2007-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  167-75     Citation Subset:  IM    
Affiliation:
Departamento de Fruticultura Tropical, Instituto Canario de Investigaciones Agrarias, Apartado 60, 38200 La Laguna, Santa Cruz de Tenerife, Spain.
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques
Coculture Techniques
Culture Media
Gene Transfer Techniques*
Genetic Markers
Genetic Vectors
Musa / cytology,  embryology,  genetics*
Rhizobium radiobacter / cytology,  genetics*
Seeds / cytology,  genetics
Soil
Transformation, Genetic*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Genetic Markers; 0/Soil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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