| Banana (Musa sp.). | |
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MedLine Citation:
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PMID: 17033061 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cultivated bananas are vegetatively propagating herbs, which are difficult to breed because of widespread male and female sterility. As a complementary gene transfer method in banana, the described Agrobacterium protocol relies on highly regenerable embryogenic cell cultures. Embryogenic cells are infected and co-cultivated in the presence of acetosyringone with Agrobacterium tumefaciens harboring a binary plasmid vector to obtain a mixed population of transformed and untransformed plant cells. Transformed plant cells are promoted to grow for 2 to 3 mo on a cell colony induction medium containing the antibiotics geneticin or hygromycin as selective agents, while agrobacteria are counterselected by timentin. The whole procedure, including plant regeneration, takes approx 6 mo and results in an average frequency of 25 to 50 independent transgenic plants per plate, which equals 50 mg of embryogenic cells. This method has been applied to a wide range of cultivars and to generate large populations of transgenic colonies and plants for tagging genes and promoters in banana. |
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Authors:
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Juan B Pérez Hernández; Serge Remy; Rony Swennen; László Sági |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 344 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2006 |
Date Detail:
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Created Date: 2006-10-11 Completed Date: 2007-02-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 167-75 Citation Subset: IM |
Affiliation:
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Departamento de Fruticultura Tropical, Instituto Canario de Investigaciones Agrarias, Apartado 60, 38200 La Laguna, Santa Cruz de Tenerife, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Culture Techniques Coculture Techniques Culture Media Gene Transfer Techniques* Genetic Markers Genetic Vectors Musa / cytology, embryology, genetics* Rhizobium radiobacter / cytology, genetics* Seeds / cytology, genetics Soil Transformation, Genetic* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Genetic Markers; 0/Soil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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