Document Detail


Balloon catheter injury abolishes phenylephrine-induced relaxation in the rat contralateral carotid.
MedLine Citation:
PMID:  21323906     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND PURPOSE: The consequences of compensatory responses to balloon catheter injury in rat carotid artery, on phenylephrine-induced relaxation and contraction in the contralateral carotid artery were studied.
EXPERIMENTAL APPROACH: Relaxation and contraction concentration-response curves for phenylephrine were obtained for contralateral carotid arteries in the presence of indomethacin (COX inhibitor), SC560 (COX-1 inhibitor), SC236 (COX-2 inhibitor) or 4-hydroxytetramethyl-L-piperidine-1-oxyl (tempol; superoxide dismutase mimetic). Reactive oxygen species were measured in carotid artery endothelial cells fluorimetrically with dihydroethidium.
KEY RESULTS: Phenylephrine-induced relaxation was abolished in contralateral carotid arteries from operated rats (E(max) = 0.01 ± 0.004 g) in relation to control (E(max) = 0.18 ± 0.005 g). Phenylephrine-induced contractions were increased in contralateral arteries (E(max) = 0.54 ± 0.009 g) in relation to control (E(max) = 0.38 ± 0.014 g). SC236 restored phenylephrine-induced relaxation (E(max) = 0.17 ± 0.004 g) and contraction (E(max) = 0.34 ± 0.018 g) in contralateral arteries. Tempol restored phenylephrine-induced relaxation (E(max) = 0.19 ± 0.012 g) and contraction (E(max) = 0.42 ± 0.014 g) in contralateral arteries, while apocynin did not alter either relaxation (E(max) = 0.01 ± 0.004 g) or contraction (E(max) = 0.54 ± 0.009 g). Dihydroethidium fluorescence was increased in contralateral samples (18 882 ± 435 U) in relation to control (10 455 ± 303 U). SC236 reduced the fluorescence in contralateral samples (8250 ± 365 U).
CONCLUSIONS AND IMPLICATIONS: Balloon catheter injury abolished phenylephrine-induced relaxation and increased phenylephrine-induced contraction in contralateral carotid arteries, through O(2) (-) derived from COX-2.
Authors:
L Pernomian; Ms Gomes; Am de Oliveira
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of pharmacology     Volume:  163     ISSN:  1476-5381     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-27     Completed Date:  2011-10-06     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  770-81     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. British Journal of Pharmacology © 2011 The British Pharmacological Society.
Affiliation:
Department of Pharmacology, School of Medicine of Ribeirão Preto, Laboratory of Pharmacology, University of São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Angioplasty, Balloon, Coronary / adverse effects*
Animals
Carotid Arteries / drug effects*,  metabolism,  physiology
Carotid Artery Injuries / metabolism,  physiopathology*
Cyclic N-Oxides / pharmacology
Cyclooxygenase Inhibitors / pharmacology
Endothelium, Vascular / drug effects,  injuries
Indomethacin / pharmacology
Male
Muscle Contraction / drug effects*
Muscle, Smooth, Vascular / drug effects,  metabolism
NADP / metabolism
NADPH Oxidase / metabolism
Oxygen / metabolism
Phenylephrine / pharmacology*
Prostaglandin-Endoperoxide Synthases / metabolism
Pyrazoles / pharmacology
Rats
Rats, Wistar
Reactive Oxygen Species / metabolism
Spin Labels
Sulfonamides / pharmacology
Vasodilation / drug effects*
Chemical
Reg. No./Substance:
0/4-(5-(4-chlorophenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl)benzenesulfonamide; 0/Cyclic N-Oxides; 0/Cyclooxygenase Inhibitors; 0/Pyrazoles; 0/Reactive Oxygen Species; 0/SC 560; 0/Spin Labels; 0/Sulfonamides; 2226-96-2/tempol; 53-59-8/NADP; 53-86-1/Indomethacin; 59-42-7/Phenylephrine; 7782-44-7/Oxygen; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases; EC 1.6.3.1/NADPH Oxidase
Comments/Corrections

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