Document Detail


Balance of Treg versus T-helper cells in the transition from symptomless to lesional psoriatic skin.
MedLine Citation:
PMID:  23330679     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: In the pathogenesis of psoriasis, pro-inflammatory T-cells are strongly involved in the inflammatory process, where regulatory T-cell (Treg) function is impaired. OBJECTIVES: As effective Treg function is associated with a numerical balance between Treg and effector T-cells, we wondered whether Treg/T-helper cell ratios may be associated with certain stages of the inflammatory process. We opted for the margin zone model as a dynamic approach. METHODS: From nine patients with chronic plaque psoriasis, 3 mm punch biopsies were obtained from the centre and margin of the lesion, perilesional skin, and distant uninvolved skin. Skin biopsies of ten healthy volunteers were included as control. Samples were analyzed by immunohistochemistry and immunefluorescence. RESULTS: In the transition from symptomless to lesional skin, a significant increase for CD3+, CD4+, and Foxp3+ cells was found. In 7/9 patients the ratio Treg (Foxp3+) versus CD4+ T-cells was higher in the distant uninvolved skin than in the perilesional and lesional skin. Interestingly, the Foxp3/CD4 ratio in the distant uninvolved skin was even higher than in the skin of healthy controls. Notably, we found that the majority of IL-17 expression was not related to CD4+ cells, but to mast cells. CONCLUSIONS: The relatively high Foxp3/CD4 ratio in symptomless skin of psoriatic patients suggests an active immune controlling mechanism distant from the psoriatic plaque. In the margin and center of the plaque the ratio appears skewed towards effector cells associated with inflammation. IL-17, an important driver of the psoriatic process, was mostly related to mast cells, and only sporadically to T-cells.
Authors:
R R M C Keijsers; H M J van der Velden; P E J van Erp; R T de Boer-van Huizen; I Joosten; H J P M Koenen; P C M van de Kerkhof
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-21
Journal Detail:
Title:  The British journal of dermatology     Volume:  -     ISSN:  1365-2133     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
©The Authors BJD © 2013 British Association of Dermatologists.
Affiliation:
Department of Dermatology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands; Department of Laboratory Medicine, Laboratory of Medical Immunology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands; Nijmegen Institute for Infection, Inflammation and Immunity (N4i), Nijmegen, The Netherlands.
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