Document Detail


Baicalein Inhibits Formation of α-Synuclein Oligomers within Living Cells and Prevents Aβ Peptide Fibrillation and Oligomerisation.
MedLine Citation:
PMID:  21271629     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abnormal protein aggregation in the brain is linked to the pathogenesis of neurodegenerative diseases, including Alzheimer's disease (AD) and Parkinson's disease (PD). Recent studies revealed that the oligomeric form of aggregates is most likely the toxic species, and thus could be a good therapeutic target. To screen for potent inhibitors that can inhibit both oligomerisation and fibrillation of α-synuclein (α-syn), we systematically compared the antioligomeric and antifibrillar activities of eight compounds that were extracted from Chinese herbal medicines through three platforms that can monitor the formation of α-syn fibrils and oligomers in cell-free or cellular systems. Our results revealed that baicalein, a flavonoid extracted from the Chinese herbal medicine Scutellaria baicalensis Georgi ("huang qin" in Chinese), is a potent inhibitor of α-syn oligomerisation both in cell-free and cellular systems, and is also an effective inhibitor of α-syn fibrillation in cell-free systems. We further tested the protective effect of baicalein against α-syn-oligomer-induced toxicity in neuronal cells. Our data showed that baicalein inhibited the formation of α-syn oligomers in SH-SY5Y and Hela cells, and protected SH-SY5Y cells from α-syn-oligomer-induced toxicity. We also explored the effect of baicalein on amyloid-β peptide (Aβ) aggregation and toxicity. We found that baicalein can also inhibit Aβ fibrillation and oligomerisation, disaggregate pre-formed Aβ amyloid fibrils and prevent Aβ fibril-induced toxicity in PC12 cells. Our study indicates that baicalein is a good inhibitor of amyloid protein aggregation and toxicity. Given the role of these processes in neurodegenerative diseases such as AD and PD, our results suggest that baicalein has potential as a therapeutic agent for the treatment of these devastating disorders.
Authors:
Jia-Hong Lu; Mustafa Taleb Ardah; Siva Sundara Kumar Durairajan; Liang-Feng Liu; Li-Xia Xie; Wang-Fun David Fong; Mohamed Y Hasan; Jian-Dong Huang; Omar M A El-Agnaf; Min Li
Related Documents :
7416969 - Processing of influenza ha protein in mdck cells: components with different mobilities ...
19308999 - Functional characterization of mesenchymal stem cells labeled with a novel pvp-coated s...
21110069 - Pten status is related to cell proliferation and self-renewal independent of cd133 phen...
11292529 - Thymidine utilization abnormality in proliferating lymphocytes and hepatocytes of the w...
8379389 - Peritoneal cell labelling: a study on the migration of macrophages and dendritic cells ...
501729 - The use of indium-111 for studies of cytotoxicity mediated by lymphocytes or by antibod...
15623789 - Trypan blue: effect on retinal pigment epithelial and neurosensory retinal cells.
22626469 - Effect of chemical peeling on the skin in relation to uv irradiation.
1478319 - Cellular automata: retinal cells, circulation and patterns.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-26
Journal Detail:
Title:  Chembiochem : a European journal of chemical biology     Volume:  -     ISSN:  1439-7633     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-1-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100937360     Medline TA:  Chembiochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
School of Chinese Medicine, Hong Kong Baptist University, No. 7 Hong Kong Baptist University Road, Kowloon Tong (Hong Kong), Fax: (852) 34112461.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Identification of CYP106A2 as a Regioselective Allylic Bacterial Diterpene Hydroxylase.
Next Document:  The Effect of Specific Adsorption of Cations and Their Size on the Charge-Compensation Mechanism in ...