Document Detail

The BaeSR regulon is involved in defense against zinc toxicity in E. coli.
MedLine Citation:
PMID:  23446818     Owner:  NLM     Status:  MEDLINE    
Intracellular zinc homeostasis is regulated by an extensive network of transporters, ligands and transcription factors. The zinc detoxification functions of three transporters and a periplasmic protein regulated by the BaeSR two-component system were explored in this work by evaluating the effect of single gene knockouts in the BaeSR regulon on the cell growth rate, free zinc, total zinc and total copper after zinc shock. Two exporters, MdtABC and MdtD, and the periplasmic protein, Spy, are involved in zinc detoxification based on the growth defects at high cell density and increases in free (>1000-fold) and total zinc/copper (>2-fold) that were observed in the single knockout strains upon exposure to zinc. These proteins complement the ATP-driven zinc export mediated by ZntA in E. coli to limit zinc toxicity. These results highlight the functions of the BaeSR regulon in metal homeostasis.
Da Wang; Carol A Fierke
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Metallomics : integrated biometal science     Volume:  5     ISSN:  1756-591X     ISO Abbreviation:  Metallomics     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-28     Completed Date:  2013-09-17     Revised Date:  2014-04-01    
Medline Journal Info:
Nlm Unique ID:  101478346     Medline TA:  Metallomics     Country:  England    
Other Details:
Languages:  eng     Pagination:  372-83     Citation Subset:  IM    
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MeSH Terms
Escherichia coli / cytology,  drug effects*,  genetics*,  growth & development
Escherichia coli Proteins / genetics*,  metabolism
Gene Deletion
Gene Expression Regulation, Bacterial / drug effects
Gene Knockout Techniques
Genes, Bacterial / genetics
Intracellular Space / drug effects,  metabolism
Protein Kinases / genetics*,  metabolism
Regulon / genetics*
Toxicity Tests*
Trans-Activators / genetics*,  metabolism
Transcription, Genetic / drug effects
Transformation, Genetic / drug effects
Zinc / toxicity*
Grant Support
Reg. No./Substance:
0/BaeR protein, E coli; 0/Escherichia coli Proteins; 0/Trans-Activators; EC 2.7.-/Protein Kinases; EC 2.7.3.-/BaeS protein, E coli; J41CSQ7QDS/Zinc

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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