| Bacteriophage phi X174-specific mRNA synthesis in cells deficient in termination factor rho activity. | |
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MedLine Citation:
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PMID: 6454004 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A previous report (Hayashi et al., Proc. Natl. Acad. Sci. U.S.A. 73:3519-3523, 1976) indicated that in vivo bacteriophage phi X174 mRNA's terminate after genes J, F, G, and H. However, termination at these sites is not stringent. To determine whether termination of phi X174 transcription depends on rho factor activity, we introduced a temperature-sensitive rho mutation (nitA) into a phi X174-sensitive host cell line and determined termination sites in wild-type and nitA cells. We found that (i) normal phi X174 terminators were recognized in phi X174-infected nitA cells, (ii) the rho mutation relieved polar effects caused by nonsense mutations in the phage genome or by chloramphenicol treatment of the host cells, and (iii) polarity was not caused by premature termination of transcription at the site of the polar mutation. RNA synthesis continued beyond the site to the first rho-sensitive site. |
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Authors:
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M N Hayashi; M Hayashi; M Imai |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 38 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1981 Apr |
Date Detail:
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Created Date: 1981-08-10 Completed Date: 1981-08-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 198-207 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriophage phi X 174
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genetics* Chloramphenicol / pharmacology Mutation RNA, Messenger / biosynthesis* RNA, Viral / biosynthesis* Rho Factor / genetics* Transcription Factors / genetics* Transcription, Genetic* |
| Grant Support | |
ID/Acronym/Agency:
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GM 12934/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/RNA, Viral; 0/Rho Factor; 0/Transcription Factors; 56-75-7/Chloramphenicol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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