Document Detail


Bacteriophage phi X174-specific mRNA synthesis in cells deficient in termination factor rho activity.
MedLine Citation:
PMID:  6454004     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A previous report (Hayashi et al., Proc. Natl. Acad. Sci. U.S.A. 73:3519-3523, 1976) indicated that in vivo bacteriophage phi X174 mRNA's terminate after genes J, F, G, and H. However, termination at these sites is not stringent. To determine whether termination of phi X174 transcription depends on rho factor activity, we introduced a temperature-sensitive rho mutation (nitA) into a phi X174-sensitive host cell line and determined termination sites in wild-type and nitA cells. We found that (i) normal phi X174 terminators were recognized in phi X174-infected nitA cells, (ii) the rho mutation relieved polar effects caused by nonsense mutations in the phage genome or by chloramphenicol treatment of the host cells, and (iii) polarity was not caused by premature termination of transcription at the site of the polar mutation. RNA synthesis continued beyond the site to the first rho-sensitive site.
Authors:
M N Hayashi; M Hayashi; M Imai
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  38     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1981 Apr 
Date Detail:
Created Date:  1981-08-10     Completed Date:  1981-08-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  198-207     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacteriophage phi X 174 / genetics*
Chloramphenicol / pharmacology
Mutation
RNA, Messenger / biosynthesis*
RNA, Viral / biosynthesis*
Rho Factor / genetics*
Transcription Factors / genetics*
Transcription, Genetic*
Grant Support
ID/Acronym/Agency:
GM 12934/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/RNA, Viral; 0/Rho Factor; 0/Transcription Factors; 56-75-7/Chloramphenicol
Comments/Corrections

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