| Bacteriophage Mu-mediated gene transposition and in vitro cloning of the enterochelin gene cluster of Escherichia coli. | |
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MedLine Citation:
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PMID: 6260579 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transposition of chromosomal genes using bacteriophage Mu has been used to obtain a partial order of the nine closely linked genes of the enterochelin-dependent iron transport system of Escherichia coli K-12. Fragments of the ent gene cluster were transposed into the conjugative plasmid RP4 and were characterized by genetic complementation. The partial gene order (entD, fes), entF, fep, entC, ent(ABEG)...lip was derived using six plasmids which carried overlapping parts of the cluster, and the fep mutations were shown to belong to a single complementation group. Two restriction fragments, one carrying ent(ABCEG) and the other carrying fep, were cloned in vitro using one of the RP4::ent plasmids as a source of DNA enriched in enterochelin system genes. A further restriction fragment, carrying the three remaining genes, entD, fes and entF was cloned directly from the chromosome. The three restriction fragments collectively cover a region of the chromosome 29 kg in length, indicating that the genes of the enterochelin system are clustered but not contiguous. |
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Authors:
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A J Laird; D W Ribbons; G C Woodrow; I G Young |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Gene Volume: 11 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 1980 Nov |
Date Detail:
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Created Date: 1981-06-13 Completed Date: 1981-06-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7706761 Medline TA: Gene Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 347-57 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriophage mu
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genetics* Cloning, Molecular DNA Restriction Enzymes DNA Transposable Elements Enterobactin / genetics* Escherichia coli / genetics* Genetic Complementation Test Mutation R Factors Recombination, Genetic Serine / analogs & derivatives* |
| Chemical | |
Reg. No./Substance:
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0/DNA Transposable Elements; 28384-96-5/Enterobactin; 56-45-1/Serine; EC 3.1.21.-/DNA Restriction Enzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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