Document Detail


Bacteriolysis of Veillonella alcalescens by lysozyme and inorganic anions present in saliva.
MedLine Citation:
PMID:  6166567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Veillonella alcalescens subsp. dispar was grown in a synthetic medium containing either radiolabeled thymidine or uridine to monitor cell lysis by assay of the release of deoxyribonucleic acid or ribonucleic acid (RNA), respectively. Biochemical analyses demonstrated that, although human or hen egg white lysozymes alone did not release deoxyribonucleic acid or RNA, the nucleic acids were liberated in equal amounts from lysozyme-treated cells by the addition of low concentrations of the sodium salts of HCO-3, SCN-, Cl-, and F-, RNA release was dependent on enzyme and anion concentration. Human lysozyme was more potent than hen egg white lysozyme, and bicarbonate was the most effective anion in promoting bacteriolysis. Surprisingly, ultrastructural analyses differed from biochemical results. Lysozyme alone caused lysis in approximately 40% of the cell population. Detailed ultrastructural examination revealed aggregated cytoplasmic components which appeared as small clumps, explaining why nucleic acids were not measurable in the biochemical assays. In reaction mixtures containing lysozyme plus inorganic salts, electron microscopy results were compatible with biochemical data. Ultrastructural studies demonstrated that the addition of inorganic salts to lysozyme-treated cells resulted in the solubilization of the protoplasmic aggregates of lysed cells, presumably freeing the complexed RNA, and in the rapid lysis of the remaining cells (approximately 60%). These data suggest that electron microscopy must be used in conjunction with biochemical assays to assess lytic damage of bacterial cells.
Authors:
M Tortosa; M I Cho; T J Wilkens; V J Iacono; J J Pollock
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  32     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1981 Jun 
Date Detail:
Created Date:  1981-09-22     Completed Date:  1981-09-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1261-73     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anions
Bacteriolysis* / drug effects
DNA, Bacterial / metabolism
Dental Plaque / microbiology
Humans
Microscopy, Electron
Muramidase / pharmacology,  physiology*
RNA, Bacterial / metabolism
Saliva / physiology*
Veillonella* / metabolism,  ultrastructure
Grant Support
ID/Acronym/Agency:
DE-04296/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Anions; 0/DNA, Bacterial; 0/RNA, Bacterial; EC 3.2.1.17/Muramidase
Comments/Corrections

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