Document Detail


Bacteriocinogenic and virulence potential of Enterococcus isolates obtained from raw milk and cheese.
MedLine Citation:
PMID:  22587647     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To provide molecular and phenotypical characterization of Enterococcus isolates obtained from raw milk and cheese, regarding their bacteriocinogenic and virulence activity.
METHODS AND RESULTS: Forty-three bacteriocinogenic enterococci isolates were identified by 16s rDNA, fingerprinted by RAPD-PCR analysis and tested by PCR for the presence of genes for lantibiotics (lanM, lanB and lanC) and enterocins (entA, entB, entP, entL50AB and entAS48) and by phenotypical methods for bacteriocin production and inhibitory spectrum. Also, the virulence of the isolates was evaluated by PCR for genes gelE, hyl, asa1, esp, cylA, efaA, ace, vanA, vanB, hdc1, hdc2, tdc and odc and by phenotypical tests for gelatinase, lipase, DNAse and α- and β-haemolysis. Most isolates (93·0%) harboured at least one lantibiotic or enterocin gene and were positive for several tested virulence genes, mainly asa1 (100%), gelE (93·0%) and efaA (83.7%). 53.5% of the isolates presented β-haemolysis [corrected].
CONCLUSIONS: Enterococcus spp. isolates presented an interesting potential application for food preservation because of bacteriocin production; however, virulence-related genes were identified in all RAPD profiles.
SIGNIFICANCE AND IMPACT OF THE STUDY: The study demonstrated the contradictory characteristics of the tested Enterococcus isolates: they presented a good potential for application in food biopreservation but contained several virulence factors.
Authors:
P M Moraes; L M Perin; S D Todorov; A Silva; B D G M Franco; L A Nero
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-11
Journal Detail:
Title:  Journal of applied microbiology     Volume:  113     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-18     Completed Date:  2013-05-22     Revised Date:  2014-01-17    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  318-28     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteriocins / genetics
Cheese / microbiology*
Enterococcus / classification*,  genetics,  isolation & purification,  pathogenicity*
Genes, Bacterial
Milk / microbiology*
RNA, Ribosomal, 16S / genetics
Random Amplified Polymorphic DNA Technique
Virulence
Chemical
Reg. No./Substance:
0/Bacteriocins; 0/RNA, Ribosomal, 16S
Comments/Corrections
Erratum In:
J Appl Microbiol. 2012 Dec;113(6):1564

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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