Document Detail


Bacteriocin production, plasmid content and plasmid location of enterocin P structural gene in enterococci isolated from food sources.
MedLine Citation:
PMID:  16599984     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To characterize bacteriocin production, antimicrobial spectrum and plasmid content in bacteriocinogenic enterococci from foods. METHODS AND RESULTS: Bacteriocinogenic Enterococcus faecium (14 isolates) and Enterococcus faecalis (three isolates) showed two different patterns of bacteriocin production in liquid broth: exponential-phase and stationary-phase production. Bacteriocin concentrates from all enterococci were inactivated by trypsin, but seldom by heat (100-117 degrees C), extremes of pH (2.0 to 9.0) or reducing agents (such as dithiothreitol). All bacteriocin concentrates were active against Listeria innocua and Listeria monocytogenes, and most were also active against many Ent. faecalis and Ent. faecium isolates. Enterococci clustered in three main groups according to their plasmid content (which included plasmids from 2.0 to 53 kb). Several isolates from different foods showed almost identical plasmid profiles. The enterocin P structural gene (entP) was detected by hybridization on plasmids of c. 19, 26 and/or 35-38 kb. CONCLUSIONS: Enterococci from food show different patterns of bacteriocin production and different plasmid content in spite of carrying similar bacteriocin-encoding genes. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides information on the diversity of bacteriocinogenic enterococci from food sources carrying apparently similar enterocin genes.
Authors:
H Abriouel; N Ben Omar; R Lucas; M Martínez-Cañamero; A Gálvez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  42     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-07     Completed Date:  2006-05-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  331-7     Citation Subset:  IM    
Affiliation:
Departamento de Ciencias de la Salud, Area de Microbiología, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaen, Spain.
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MeSH Terms
Descriptor/Qualifier:
Bacteriocins / biosynthesis*,  genetics
Enterococcus faecalis / drug effects,  genetics,  metabolism*
Enterococcus faecium / drug effects,  genetics,  metabolism*
Food Microbiology*
Plasmids*
Chemical
Reg. No./Substance:
0/Bacteriocins; 0/enterocin P

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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